Multiplex PCR Assay Targeting a Diguanylate Cyclase-Encoding Gene, cgcA, To Differentiate Species within the Genus Cronobacter

被引：46

作者：

Carter, L. ^{[1
]}

Lindsey, L. A. ^{[1
]}

Grim, C. J. ^{[1
,2
]}

Sathyamoorthy, V. ^{[1
]}

Jarvis, K. G. ^{[1
]}

Gopinath, G. ^{[1
]}

Lee, C. ^{[1
]}

Sadowski, J. A. ^{[1
]}

Trach, L. ^{[1
]}

Pava-Ripoll, M. ^{[3
]}

McCardell, B. A. ^{[1
]}

Tall, B. D. ^{[1
]}

Hu, L. ^{[1
,2
]}

机构：

[1] US FDA, CFSAN, Laurel, MD USA

[2] Oak Ridge Inst Sci & Educ, Oak Ridge, TN USA

[3] US FDA, CFSAN, College Pk, MD USA

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2013年 / 79卷 / 02期

关键词：

ENTEROBACTER-SAKAZAKII; SEQUENCE;

D O I：

10.1128/AEM.02898-12

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

In a comparison to the widely used Cronobacter rpoB PCR assay, a highly specific multiplexed PCR assay based on cgcA, a diguanylate cyclase gene, that identified all of the targeted six species among 305 Cronobacter isolates was designed. This assay will be a valuable tool for identifying suspected Cronobacter isolates from food-borne investigations.

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收藏

页码：734 / 737

页数：4

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