Molecular characterizations of two grapevine rupestris stem pitting-associated virus isolates from China

被引:17
作者
Hu, Guo-Jun [1 ]
Dong, Ya-Feng [1 ]
Zhu, Hong-Juan [1 ]
Zhang, Zun-Ping [1 ]
Fan, Xu-Dong [1 ]
Ren, Fang [1 ]
Zhou, Jun [1 ]
机构
[1] Chinese Acad Agr Sci, Res Inst Pomol, Natl Ctr Eliminating Viruses Deciduous Fruit Tree, Xingcheng 125100, Peoples R China
关键词
NUCLEOTIDE-SEQUENCE; GENETIC DIVERSITY; GENOME STRUCTURE; RT-PCR; FOVEAVIRUS; RECOMBINATION; LOCALIZATION; VARIANTS; PROTEIN; CLONES;
D O I
10.1007/s00705-015-2544-6
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The complete nucleotide sequences of two isolates of grapevine rupestris stem pitting-associated virus (LSL and JF) collected from grapevine of Xingcheng in Liaoning Province, China, were determined. The genomes of both LSL and JF were found to contain five open reading frames (ORFs). Sequence alignments showed that the genomic sequences of JF were 76.1 %-83.5 % identical to the other ten GRSPaV isolates that have been reported previously and that the nucleotide sequence identity of isolate LSL to other isolates was no more than 78 %. Phylogenetic analysis based on the complete genome sequence indicated that JF belongs to group III and that LSL belongs to a new group (group IV). The average genetic distances of the new genetic lineage from groups I, II and III were 0.34, 0.32 and 0.33, respectively.
引用
收藏
页码:2641 / 2645
页数:5
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