Structural characterization of nitric oxide-bound soluble Guanylate Cyclase using resonance Raman spectroscopy

被引：1

作者：

Pal, Biswajit ^{[1
]}

Tanaka, Katsuhiro ^{[2
]}

Takenaka, Shigeo ^{[2
]}

Shaik, Tajith B. ^{[1
]}

Kitagawa, Teizo ^{[3
]}

机构：

[1] CSIR, Ctr Cellular & Mol Biol, Hyderabad 500007, Andhra Pradesh, India

[2] Osaka Prefecture Univ, Dept Vet Sci, Sakai, Osaka 5938531, Japan

[3] Univ Hyogo, Grad Sch Life Sci, Picobiol Inst, Kamigori, Hyogo 6781297, Japan

来源：

JOURNAL OF PORPHYRINS AND PHTHALOCYANINES | 2013年 / 17卷 / 03期

关键词：

soluble Guanylate Cyclase; resonance Raman spectroscopy; GTP; YC-1; BAY; nitric oxide; ACTIVATION; HEME; BINDING; NO; MYOGLOBIN; YC-1; IDENTIFICATION; MECHANISM; RESIDUES; DEUTERATION;

D O I：

10.1142/S1088424613500375

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Mammalian soluble Guanylate Cyclase (sGC), working as a physiological NO receptor, is investigated using resonance Raman spectroscopy for NO bound states with different saturation levels in the presence and absence of effectors. The Fe-NO (nu(Fe-NO)) and N-O (nu(N-O)) stretching bands appeared at 521 and 1681 cm(-1), respectively, without effectors, but nu(N-O) was split into 1681 and 1699 cm(-1) in the presence of GTP and shifted to 1687 cm(-1) in the presence of YC-1 or BAY 41-2272, while nu(Fe-NO) remained unaltered. The split two nu(N-O) bands were independent of NO saturation levels. GTP or YC-1/BAY 41-2272 altered the vinyl and propionate bending modes from 423 to 399 cm(-1) and 376 to 367 cm(-1), respectively. Based on these observations, allosteric effects on NO ... protein interactions are discussed.

引用

页码：240 / 246

页数：7

共 46 条

[1] Revisiting the kinetics of nitric oxide (NO) binding to soluble guanylate cyclase: The simple NO-binding model is incorrect [J].

Ballou, DP ;

Zhao, Y ;

Brandish, PE ;

Marletta, MA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (19) :12097-12101

[2] On the activation of soluble guanylyl cyclase by nitric oxide [J].

Bellamy, TC ;

Wood, J ;

Garthwaite, J .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (01) :507-510

[3] A molecular basis for NO selectivity in soluble guanylate cyclase [J].

Boon, EM ;

Huang, SH ;

Marletta, MA .

NATURE CHEMICAL BIOLOGY, 2005, 1 (01) :53-59

[4] NITRIC-OXIDE - A PHYSIOLOGICAL MESSENGER MOLECULE [J].

BREDT, DS ;

SNYDER, SH .

ANNUAL REVIEW OF BIOCHEMISTRY, 1994, 63 :175-195

[5] Tonic and acute nitric oxide signaling through soluble guanylate cyclase is mediated by nonheme nitric oxide, ATP, and GTP [J].

Cary, SPL ;

Winger, JA ;

Marletta, MA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2005, 102 (37) :13064-13069

[6] VINYL INFLUENCES ON PROTOHEME RESONANCE RAMAN-SPECTRA - NICKEL(II) PROTOPORPHYRIN-IX WITH DEUTERATED VINYL GROUPS [J].

CHOI, S ;

SPIRO, TG ;

LANGRY, KC ;

SMITH, KM .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1982, 104 (16) :4337-4344

[7] Interaction of soluble guanylate cyclase with YC-1: Kinetic and resonance Raman studies [J].

Denninger, JW ;

Schelvis, JPM ;

Brandish, PE ;

Zhao, Y ;

Babcock, GT ;

Marletta, MA .

BIOCHEMISTRY, 2000, 39 (14) :4191-4198

[8] Characterization of two different five-coordinate soluble guanylate cyclase ferrous-nitrosyl complexes [J].

Derbyshire, Emily R. ;

Gunn, Alexander ;

Ibrahim, Mohammed ;

Spiro, Thomas G. ;

Britt, R. David ;

Marletta, Michael A. .

BIOCHEMISTRY, 2008, 47 (12) :3892-3899

[9] Butyl isocyanide as a probe of the activation mechanism of soluble guanylate cyclase [J].

Derbyshire, Emily R. ;

Marletta, Michael A. .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2007, 282 (49) :35741-35748

[10] Incorporation of Tyrosine and Glutamine Residues into the Soluble Guanylate Cyclase Heme Distal Pocket Alters NO and O2 Binding [J].

Derbyshire, Emily R. ;

Deng, Sarah ;

Marletta, Michael A. .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2010, 285 (23) :17471-17478

← 1 2 3 4 5 →