Type 2 diabetes mellitus alters cardiac mitochondrial content and function in a non-obese mice model

被引：1

作者：

De Yurre, Ainhoa R. ^{[1
]}

Martins, Eduarda G. L. ^{[1
,2
]}

Lopez-Alarcon, Micaela ^{[1
]}

Cabral, Bruno ^{[1
]}

Vera, Narendra ^{[1
]}

Lopes, Jarlene A. ^{[1
]}

Galina, Antonio ^{[2
]}

Takiya, Christina M. ^{[1
]}

Lindoso, Rafael S. ^{[1
]}

Vieyra, Adalberto ^{[3
,4
]}

Saenz, Oscar C. ^{[5
]}

Medei, Emiliano ^{[1
,4
]}

机构：

[1] Univ Fed Rio de Janeiro, Inst Biofis Carlos Chagas Filho, Ave Carlos Chagas Filho 373, BR-21941902 Rio De Janeiro, RJ, Brazil

[2] Univ Fed Rio de Janeiro, Inst Bioquim Med Leopoldo Meis, Ave Carlos Chagas Filho 373, BR-21941902 Rio De Janeiro, RJ, Brazil

[3] Univ Grande Rio, Programa Posgrad Biomed Translac, Rua Prof Jose de Souza Herdy 1160, BR-25071202 Duque De Caxias, RJ, Brazil

[4] Univ Fed Rio de Janeiro, Ctr Nacl Biol Estrutural & Bioimagem CENABIO, Ave Carlos Chagas Filho 373, BR-21941902 Rio De Janeiro, RJ, Brazil

[5] Univ Basque Country, UPV EHU, Fac Farm, Dept Fisiol, Paseo Univ 7, Vitoria 01006, Spain

来源：

ANAIS DA ACADEMIA BRASILEIRA DE CIENCIAS | 2020年 / 92卷 / 02期

关键词：

Heart; high fat diet; mitochondria; streptozotocin; Type; 2; diabetes; HIGH-FAT DIET; ANIMAL-MODELS; RAT; DYSFUNCTION; DISEASE; FIBERS; RISK;

D O I：

10.1590/0001-3765202020191340

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Type 2 diabetes mellitus (T2DM) is associated with an increase of premature appearance of several disorders such as cardiac complications. Thus, we test the hypothesis that a combination of a high fat diet (HFD) and low doses of streptozotocin (STZ) recapitulate a suitable mice model of T2DM to study the cardiac mitochondrial disturbances induced by this disease. Animals were divided in 2 groups: the T2DM group was given a HFD and injected with 2 low doses of STZ, while the CNTRL group was given a standard chow and a buffer solution. The combination of HFD and STZ recapitulate the T2DM metabolic profile showing higher blood glucose levels in T2DM mice when compared to CNTRL, and also, insulin resistance. The kidney structure/function was preserved. Regarding cardiac mitochondrial function, in all phosphorylative states, the cardiac mitochondria from T2DM mice presented reduced oxygen fluxes when compared to CNTRL mice. Also, mitochondria from T2DM mice showed decreased citrate synthase activity and lower protein content of mitochondrial complexes. Our results show that in this non-obese T2DM model, which recapitulates the classical metabolic alterations, mitochondrial function is impaired and provides a useful model to deepen study the mechanisms underlying these alterations.

引用

页码：1 / 15

页数：15

共 40 条

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