Slower growth ofEscherichia colileads to longer survival in carbon starvation due to a decrease in the maintenance rate

被引:69
作者
Biselli, Elena [1 ]
Schink, Severin Josef [1 ,2 ]
Gerland, Ulrich [1 ]
机构
[1] Tech Univ Munich, Dept Phys, Phys Complex Biosyst, Garching, Germany
[2] Harvard Med Sch, Dept Syst Biol, Boston, MA 02115 USA
关键词
bacterial fitness; bacterial survival; bacterial systems biology; death rate; quantitative physiology; ESCHERICHIA-COLI; STATIONARY-PHASE; PROTEIN-PRODUCTION; AMINO-ACID; LIFE; EVOLUTION; BACTERIA; RPOS; METABOLISM; INDUCTION;
D O I
10.15252/msb.20209478
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Fitness of bacteria is determined both by how fast cells grow when nutrients are abundant and by how well they survive when conditions worsen. Here, we study how prior growth conditions affect the death rate ofEscherichia coliduring carbon starvation. We control the growth rate prior to starvation either via the carbon source or via a carbon-limited chemostat. We find a consistent dependence where death rate depends on the prior growth conditions only via the growth rate, with slower growth leading to exponentially slower death. Breaking down the observed death rate into two factors, maintenance rate and recycling yield, reveals that slower growing cells display a decreased maintenance rate per cell volume during starvation, thereby decreasing their death rate. In contrast, the ability to scavenge nutrients from carcasses of dead cells (recycling yield) remains constant. Our results suggest a physiological trade-off between rapid proliferation and long survival. We explore the implications of this trade-off within a mathematical model, which can rationalize the observation that bacteria outside of lab environments are not optimized for fast growth.
引用
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页数:13
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