Mesorhizobium atlanticum sp. nov., a new nitrogen-fixing species from soils of the Brazilian Atlantic Forest biome

被引:16
作者
Ferraz Helene, Luisa Caroline [1 ,2 ,3 ]
Dall'Agnol, Rebeca Fuzinatto [3 ]
Marcon Delamuta, Jakeline Renata [1 ,4 ]
Hungria, Mariangela [1 ,2 ,4 ]
机构
[1] Embrapa Soja, CP 231, BR-86001970 Londrina, Parana, Brazil
[2] Univ Estadual Londrina, Dept Biotechnol, CP 10011, BR-86057970 Londrina, Parana, Brazil
[3] SBN, Coordenacao Aperfeicoamento Pessoal Nivel Super, Quadra 2,BlocoL,Lote 06,Edificio Capes, BR-70040020 Brasilia, DF, Brazil
[4] Conselho Nacl Desenvolvimento Cientif & Tecnol, SHIS QI 1 ConjuntoB,Blocos A,B,C & D, BR-71605001 Brasilia, DF, Brazil
关键词
Mesorhizobium; biological nitrogen fixation; MLSA; ANI; nodulation; DNA-DNA HYBRIDIZATION; NODULES; RHIZOBIA;
D O I
10.1099/ijsem.0.003397
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Biological nitrogen fixation performed by diazotrophic bacteria is a vital process for agricultural and environmental sustainability. In recent years, bacterial classification has been based on genomic data, accelerating our understanding about the diversity, and resulting in the description of several new species. In this study, four strains (CNPSo 3140(T), CNPSo 3235, CNPSo 3236 and CNPSo 3237) trapped by Phaseolus vulgaris and Mimosa pudica from soil samples of the Brazilian Atlantic Forest biome (Mata Atlentica) were submitted to polyphasic analysis to investigate their proper classification within the genus Mesorhizobium. The 16S rRNA gene phylogram showed that the strains present sequences identical to those of Mesorhizobium acaciae and Mesorhizobium plurifarium, not allowing a clear taxonomic classification; however, when using multilocus sequence analysis methodology, the strains were grouped into a well-supported distinct Glade, with <94.5 % nucleotide identity with the other species of the genus. The average nucleotide identity of CNPSo 3140(T) genome showed values below the threshold in relation to the closest species, of 89.75 % with Mesorhizobium plurifarium and of 88.83 % with Mesorhizobium hawassense; the digital DNA-DNA hybridization values were 39 and 37.70 % with the same species, respectively. Nodulation gene (nodC) phylogeny positioned the strains in an isolated cluster, showing greater similarity to Mesorhizobium shonense. All data obtained in this study support the description of the novel species Mesorhizobium atlanticum sp. nov. The type strain is CNPSo 3140(T) (=ABIP 206(T) =LMG 30305(T) =U1602(T)), isolated from a nodule of Phaseolus vulgaris.
引用
收藏
页码:1800 / 1806
页数:7
相关论文
共 30 条
[1]   EXOPOLYSACCHARIDES PRODUCED BY THE SYMBIOTIC NITROGEN-FIXING BACTERIA OF LEGUMINOSAE [J].
Bomfeti, Cleide Aparecida ;
Florentino, Ligiane Aparecida ;
Guimaraes, Ana Paula ;
Cardoso, Patricia Gomes ;
Guerreiro, Mario Cesar ;
de Souza Moreira, Fatima Maria .
REVISTA BRASILEIRA DE CIENCIA DO SOLO, 2011, 35 (03) :657-671
[2]   Mesorhizobium camelthorni sp. nov., isolated from Alhagi sparsifolia [J].
Chen, Wei-Min ;
Zhu, Wen-Fei ;
Bontemps, Cyril ;
Young, J. Peter W. ;
Wei, Ge-Hong .
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2011, 61 :574-579
[3]   Genetic diversity of symbiotic Paraburkholderia species isolated from nodules of Mimosa pudica (L.) and Phaseolus vulgaris (L.) grown in soils of the Brazilian Atlantic Forest (Mata Atlantica) [J].
Dall' Agnol, Rebeca Fuzinatto ;
Bournaud, Caroline ;
de Faria, Sergio Miana ;
Bena, Gilles ;
Moulin, Lionel ;
Hungria, Mariangela .
FEMS MICROBIOLOGY ECOLOGY, 2017, 93 (04)
[4]   Characterization of tropical tree rhizobia and description of Mesorhizobium plurifarium sp. nov. [J].
de Lajudie, P ;
Willems, A ;
Nick, G ;
Moreira, F ;
Molouba, F ;
Hoste, B ;
Torck, U ;
Neyra, M ;
Collins, MD ;
Lindström, K ;
Dreyfus, B ;
Gillis, M .
INTERNATIONAL JOURNAL OF SYSTEMATIC BACTERIOLOGY, 1998, 48 :369-382
[5]   Mesorhizobium calcicola sp nov., Mesorhizobium waitakense sp nov., Mesorhizobium sophorae sp nov., Mesorhizobium newzealandense sp nov and Mesorhizobium kowhaii sp nov isolated from Sophora root nodules [J].
De Meyer, Sofie E. ;
Tan, Heng Wee ;
Andrews, Mitchell ;
Heenan, Peter B. ;
Willems, Anne .
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2016, 66 :786-795
[6]   MUSCLE: multiple sequence alignment with high accuracy and high throughput [J].
Edgar, RC .
NUCLEIC ACIDS RESEARCH, 2004, 32 (05) :1792-1797
[7]  
FELSENSTEIN J, 1985, EVOLUTION, V39, P783, DOI 10.1111/j.1558-5646.1985.tb00420.x
[8]   Bradyrhizobium mercantei sp nov., a nitrogen-fixing symbiont isolated from nodules of Deguelia costata (syn. Lonchocarpus costatus) [J].
Ferraz Helene, Luisa Caroline ;
Marcon Delamuta, Jakeline Renata ;
Ribeiro, Renan Augusto ;
Hungria, Mariangela .
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2017, 67 (06) :1827-1834
[9]   DNA-DNA hybridization values and their relationship to whole-genome sequence similarities [J].
Goris, Johan ;
Konstantinidis, Konstantinos T. ;
Klappenbach, Joel A. ;
Coenye, Tom ;
Vandamme, Peter ;
Tiedje, James M. .
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2007, 57 :81-91
[10]   Mesorhizobium caraganae sp nov., a novel rhizobial species nodulated with Caragana spp. in China [J].
Guan, Su Hua ;
Chen, Wen Feng ;
Wang, En Tao ;
Lu, Yang Li ;
Yan, Xue Rui ;
Zhang, Xiao Xia ;
Chen, Wen Xin .
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2008, 58 :2646-2653