Role of microRNA-27a in myoblast differentiation

被引：29

作者：

Chen, Xiaoling ^{[1
]}

Huang, Zhiqing ^{[1
]}

Chen, Daiwen ^{[1
]}

Yang, Ting ^{[1
]}

Liu, Guangmang ^{[1
]}

机构：

[1] Sichuan Agr Univ, Inst Anim Nutr, China Minist Educ, Key Lab Anim Dis Resistance Nutr, Chengdu 611130, Sichuan, Peoples R China

来源：

CELL BIOLOGY INTERNATIONAL | 2014年 / 38卷 / 02期

基金：

中国国家自然科学基金;

关键词：

microRNA; miR-27a; myoblast differentiation; myostatin; GROWTH-FACTOR-BETA; SKELETAL-MUSCLE; MYOSTATIN EXPRESSION; CELL-PROLIFERATION; MYOGENESIS; GENE; TARGETS; MYOD; TRANSCRIPTION; VERTEBRATES;

D O I：

10.1002/cbin.10192

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

MicroRNAs (miRNAs) are a class of endogenous non-coding RNAs that are critically involved in roles in various aspects of skeletal myogenesis. microRNA miR-27a promotes myoblast proliferation by targeting myostatin, a critical inhibitor of skeletal muscle development, but its mode of action in myoblast differentiation remains unclear. We have found that expression of miR-27a and myostatin were upregulated and downregulated, respectively, during myoblast differentiation. Overexpression of miR-27a increased the number of myosin heavy chain (MHC)-positive cells and upregulated mRNA and protein of MyoD and myogenin. These findings indicate that miR-27a plays a role in enhancing myoblast differentiation.

引用

页码：266 / 271

页数：6

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