Regulation of osteoclastogenesis by ganoderic acid DM isolated from Ganoderma lucidum

被引：47

作者：

Miyamoto, Ichiko

Liu, Jie

Shimizu, Kuniyoshi

Sato, Masao

Kukita, Akiko ^{[3
]}

Kukita, Toshio ^{[2
]}

Kondo, Ryuichiro ^{[1
]}

机构：

[1] Kyushu Univ, Fac Agr, Dept Forest & Forest Prod Sci, Higashi Ku, Fukuoka 8128581, Japan

[2] Kyushu Univ, Fac Dent Sci, Higashi Ku, Fukuoka 8128581, Japan

[3] Saga Univ, Fac Med, Saga 8498501, Japan

来源：

EUROPEAN JOURNAL OF PHARMACOLOGY | 2009年 / 602卷 / 01期

关键词：

Ganoderma lucidum; Bone protection; Osteoclast; Ganoderic acid DM; Triterpenoid; BREAST-CANCER; OVARIECTOMIZED RATS; MACROPHAGE FUSION; GIANT-CELLS; BONE LOSS; IN-VITRO; DC-STAMP; GENISTEIN; OSTEOPOROSIS; MECHANISM;

D O I：

10.1016/j.ejphar.2008.11.005

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

The preventative effects of the ethanol extracts of Ganoderma lucidum against the ovariectomized (Ovx)induced deterioration of bone density in 11-week-old female Sprague Dawley (SD) rats were investigated. The results showed that the G. lucidum-treated Ovx rats showed improved bone density compared with the Ovx rats. We studied the effects of G. lucidum on osteoclastic differentiation using bone marrow cells and RAW 264 cell D-clone (RAW-D). Differentiation, in response to receptor activator of NF-KB ligand (RANKL) and a tumor necrosis factor at (TNF-alpha), was inhibited by the ethanol extracts of G. lucidum and ganoderic acid DM which was isolated as one of the active compounds by bioassay-guided fractionation. Ganoderic acid DM especially suppresses the expression of c-Fos and nuclear factor of activated T cells cl (NFATc1). This suppression leads to the inhibition of dendritic cell-specific transmembrane protein (DC-STAMP) expression and reduces osteoclast fusion. (C) 2008 Published by Elsevier B.V.

引用

页码：1 / 7

页数：7

共 26 条

[21] Osteoclasts and giant cells: macrophage-macrophage fusion mechanism [J].