A facile electrochemical uricase biosensor designed from gold/amino acid nanocomposites

被引：22

作者：

Liu, Yan ^{[1
]}

Yuan, Min ^{[1
]}

Liu, Lili ^{[1
]}

Guo, Rong ^{[1
]}

机构：

[1] Yangzhou Univ, Sch Chem & Chem Engn, Yangzhou 225002, Jiangsu, Peoples R China

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2013年 / 176卷

关键词：

Gold nanoparticles; Amino acid; Biosensor; Uricase; Uric acid; GOLD NANOPARTICLES; ELECTRODE; OXIDASE; IMMOBILIZATION; ENZYMES; SERUM;

D O I：

10.1016/j.snb.2012.08.058

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A facile amperometric uric acid sensor was constructed by immobilizing uricase on gold/amino acid nanocomposites. The resultant biosensor had a short response time of less than 4 s, a sensitivity of 108 mu A mM(-1) cm(-2), and a wide linear range from 0.02 to 2.5 mM. Its experimental detection limit is 7.0 mu M (S/N = 3) and the apparent Michaelis-Menten constant is calculated to be 1.78 mM. Furthermore, it has been revealed that the biosensor exhibits excellent thermal stability, good anti-interference ability and favorable stability over relatively long-term storage. The results show that gold/amino acid nanocomposites can provide a promising material for biosensor design and other biological applications. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：592 / 597

页数：6

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