A physical map of the Chinese chestnut (Castanea mollissima) genome and its integration with the genetic map

被引：27

作者：

Fang, Guang-Chen ^{[1
]}

Blackmon, Barbara P. ^{[2
]}

Staton, Margaret E. ^{[2
]}

Nelson, C. Dana ^{[3
]}

Kubisiak, Thomas L. ^{[3
]}

Olukolu, Bode A. ^{[4
]}

Henry, David ^{[5
]}

Zhebentyayeva, Tatyana ^{[6
]}

Saski, Christopher A. ^{[2
]}

Cheng, Chun-Huai ^{[7
]}

Monsanto, Megan ^{[8
,9
]}

Ficklin, Stephen ^{[7
]}

Atkins, Michael ^{[2
]}

Georgi, Laura L. ^{[10
]}

Barakat, Abdelali ^{[12
]}

Wheeler, Nicholas ^{[11
]}

Carlson, John E. ^{[12
]}

Sederoff, Ronald ^{[13
]}

Abbott, Albert G. ^{[6
]}

机构：

[1] USDA APHIS BRS, Raleigh, NC 27606 USA

[2] Clemson Univ, Genom Inst, BRC, Clemson, SC 29634 USA

[3] US Forest Serv, Southern Inst Forest Genet, Saucier, MS 39574 USA

[4] N Carolina State Univ, Dept Plant Pathol, Raleigh, NC 27695 USA

[5] Piedmont Tech Coll, Greenwood, SC 29648 USA

[6] Clemson Univ, Dept Biochem & Genet, Clemson, SC 29634 USA

[7] Washington State Univ, Dept Hort, Pullman, WA 99164 USA

[8] San Diego State Univ, SDSU Heart Inst, San Diego, CA 92182 USA

[9] San Diego State Univ, Dept Biol, San Diego, CA 92182 USA

[10] Amer Chestnut Fdn, Meadowview, VA 24361 USA

[11] N Carolina State Univ, Dept Forestry & Environm Resources, Raleigh, NC 27695 USA

[12] Penn State Univ, Huck Inst Life Sci, Sch Forest Resources, University Pk, PA 16802 USA

[13] N Carolina State Univ, Forest Biotechnol Grp, Dept Forestry & Environm Resources, Raleigh, NC 27695 USA

来源：

TREE GENETICS & GENOMES | 2013年 / 9卷 / 02期

基金：

美国国家科学基金会;

关键词：

Castanea spp; Chestnut; BAC library; HICF fingerprinting; Physical map; Comparative genomics; Chestnut blight resistance; BIOLOGICAL-CONTROL; BLIGHT; EVOLUTION; DUPLICATION; PARASITICA; RESISTANCE; FAGACEAE; TOOLS; GENUS;

D O I：

10.1007/s11295-012-0576-6

中图分类号：

S7 [林业];

学科分类号：

0829 ; 0907 ;

摘要：

Three Chinese chestnut bacterial artificial chromosome (BAC) libraries were developed and used for physical map construction. Specifically, high information content fingerprinting was used to assemble 126,445 BAC clones into 1,377 contigs and 12,919 singletons. Integration of the dense Chinese chestnut genetic map with the physical map was achieved via high-throughput hybridization using overgo probes derived from sequence-based genetic markers. A total of 1,026 probes were anchored to the physical map including 831 probes corresponding to 878 expressed sequence tag-based markers. Within the physical map, three BAC contigs were anchored to the three major fungal blight-resistant quantitative trait loci on chestnut linkage groups B, F, and G. A subset of probes corresponding to orthologous genes in poplar showed only a limited amount of conserved gene order between the poplar and chestnut genomes. The integrated genetic and physical map of Chinese chestnut is available at www.fagaceae.org/physical_maps.

引用

页码：525 / 537

页数：13

共 44 条

[1] Polyploidy and genome evolution in plants [J].