Interaction of coagulase-negative Staphylococcus species with bovine mammary epithelial cells

被引:52
作者
Almeida, RA [1 ]
Oliver, SP [1 ]
机构
[1] Univ Tennessee, Inst Agr, Dept Anim Sci, Food Safety Ctr Excellence, Knoxville, TN 37996 USA
关键词
CNS; internalization; signal transduction; pathogenesis;
D O I
10.1006/mpat.2001.0465
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Three coagulase-negative Staphylococcus species (CNS) (Staphylococcus epidermidis, Staphylococcus xylosus and Staphylococcus hyicus), from the milk of cows with mastitis, were used to evaluate adherence to and internalization by bovine mammary epithelia[ cells, and to investigate involvement of host cell signal transduction and host cell cytoskeleton rearrangement on internalization of CNS. S. xylosus showed highest adherence and internalization values of the species evaluated. Host cell cytoskeleton polymerization and protein kinase (PK) phosphorylation were required for internalization of CNS. Both protein kinase C (PKC) and tyrosine kinase (TPK) pathways were involved, but internalization of S. xylosus occurred preferentially through epidermal growth factor TPK activity. S. epidermidis and S. hyicus seemed to exploit other TPK pathways. Results of this study showed that S. xylosus, S. hyicus and S. epidermidis were able to adhere and internalize bovine mammary cells in a process that appeared to be receptor(s) mediated and exploited host signal transduction and cytoskeleton to induce an uptake signal. (C) 2001 Academic Press.
引用
收藏
页码:205 / 212
页数:8
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