Modified Cangfu Daotan decoction ameliorates polycystic ovary syndrome with insulin resistance via NF-κB/LCN-2 signaling pathway in inflammatory microenvironment

被引:11
作者
Liu, Shuowen [1 ]
Zhang, Yao [1 ]
Yang, Fang [1 ]
Gu, Jingna [1 ]
Zhang, Ruyue [1 ]
Kuang, Yingying [1 ]
Mai, Wantong [1 ]
Zheng, Chengbo [1 ]
Yu, Yang [1 ]
Lu, Ruling [2 ]
Zeng, Lei [2 ]
Cao, Hongying [1 ]
Long, Yongling [1 ]
机构
[1] Guangzhou Univ Chinese Med, Sch Pharmaceut Sci, Guangzhou, Guangdong, Peoples R China
[2] Guangzhou Univ Chinese Med, Dept Gynecol, Affiliated Hosp 1, Guangzhou, Peoples R China
基金
中国国家自然科学基金;
关键词
Lcn-2; NF-kB signaling pathway; INSR; IRS-1; GLUT4; PCOS-IR; Modified Cangfu Daotan decoction; HORMONE-BINDING GLOBULIN; HDL-C; LIPOCALIN-2; OBESITY; HYPERANDROGENISM; METFORMIN; MARKER; WOMEN;
D O I
10.3389/fendo.2022.975724
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
This study explored the possible connection between the insulin resistance-targeting protein adipokine lipocalin-2 (LCN-2) and NF-kappa B signaling pathway in the inflammatory microenvironment in PCOS-IR model rats to determine the pharmacological mechanism of modified Cangfu Daotan decoction (MCDD) intervention for PCOS-IR. We used a high-fat diet (42 days) combined with letrozole (1 mg/kg/day, 42 days) to establish a PCOS-IR rat model. From the third week after modeling, the rats were given continuous administration of MCDD (high dose with 31.68 g/kg, medium dose with 15.84 g/kg, and low dose with 7.92 g/kg) for 28 days. Serum, ovarian tissue, liver, and adipose tissue were collected after the last gavage. Enzyme-linked immunosorbent assay, hematoxylin-eosin (HE) staining, Masson staining, qRT-PCR, and Western blot experiments were performed to detect various indicators. Our results showed that MCDD could reduce body weight and abdominal fat weight; restore normal estrous cycle and ovarian function; alleviate fatty liver; regulate HOMA-IR and OGTT index; reduce serum inflammatory factor levels, LCN-2 level, and gene expression; and regulate the insulin signal transduction and NF-kappa B pathways in PCOS-IR rats. Thus, MCDD may play a role in improving ovarian function in PCOS-IR rats by downregulating NF-kappa B/LCN-2 proteins and upregulating the gene expression of Insr/Irs-1/Glut4 in the insulin signaling pathway in the inflammatory environment.
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页数:18
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