Bacterial genome sequencing in the clinic: bioinformatic challenges and solutions

被引：101

作者：

Fricke, W. Florian ^{[1
]}

Rasko, David A. ^{[1
]}

机构：

[1] Univ Maryland, Sch Med, Dept Microbiol & Immunol, Inst Genome Sci, Baltimore, MD 21201 USA

来源：

NATURE REVIEWS GENETICS | 2014年 / 15卷 / 01期

基金：

美国国家卫生研究院;

关键词：

TOF MASS-SPECTROMETRY; CLOUD; TUBERCULOSIS; OUTBREAK; FUTURE; GALAXY;

D O I：

10.1038/nrg3624

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The potential of bacterial whole-genome sequencing (WGS) to complement existing diagnostic infrastructures in clinical microbiology has been shown in proof-of-principle examples and extensively discussed. However, less attention has been drawn to bioinformatic challenges that are associated with the clinical adoption of WGS-based molecular diagnostics. This Perspective article discusses questions that are related to standard operating procedures, computational resource management, and data storage and integration in the context of recent developments in the sequencing and bioinformatics service markets.

引用

页码：49 / 55

页数：7

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