A Functional Genomics Approach Reveals CHE as a Component of the Arabidopsis Circadian Clock

被引:371
|
作者
Pruneda-Paz, Jose L. [1 ]
Breton, Ghislain [1 ]
Para, Alessia [1 ]
Kay, Steve A. [1 ]
机构
[1] Univ Calif San Diego, Div Biol Sci, Sect Cell & Dev Biol, La Jolla, CA 92093 USA
关键词
GENE-EXPRESSION; RHYTHMS; TOC1; PLANTS; REGULATOR; GROWTH;
D O I
10.1126/science.1167206
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Transcriptional feedback loops constitute the molecular circuitry of the plant circadian clock. In Arabidopsis, a core loop is established between CCA1 and TOC1. Although CCA1 directly represses TOC1, the TOC1 protein has no DNA binding domains, which suggests that it cannot directly regulate CCA1. We established a functional genomic strategy that led to the identification of CHE, a TCP transcription factor that binds specifically to the CCA1 promoter. CHE is a clock component partially redundant with LHY in the repression of CCA1. The expression of CHE is regulated by CCA1, thus adding a CCA1/CHE feedback loop to the Arabidopsis circadian network. Because CHE and TOC1 interact, and CHE binds to the CCA1 promoter, a molecular linkage between TOC1 and CCA1 gene regulation is established.
引用
收藏
页码:1481 / 1485
页数:5
相关论文
共 50 条
  • [21] THE GENETICS OF CIRCADIAN CLOCK REGULATION IN ARABIDOPSIS
    MILLAR, AJ
    STRAUME, M
    CARRE, IA
    STRAYER, CA
    CHORY, J
    CHUA, NH
    KAY, SA
    JOURNAL OF CELLULAR BIOCHEMISTRY, 1995, : 158 - 158
  • [22] Putative components of the Arabidopsis circadian clock
    Carré, IA
    BIOLOGICAL RHYTHM RESEARCH, 1999, 30 (03) : 259 - 263
  • [23] A Dual sgRNA Approach for Functional Genomics in Arabidopsis thaliana
    Pauwels, Laurens
    De Clercq, Rebecca
    Goossens, Jonas
    Inigo, Sabrina
    Williams, Clara
    Ron, Mily
    Britt, Anne
    Goossens, Alain
    G3-GENES GENOMES GENETICS, 2018, 8 (08): : 2603 - 2615
  • [24] Temperature entrainment of the Arabidopsis circadian clock
    Eckardt, NA
    PLANT CELL, 2005, 17 (03): : 645 - 647
  • [25] Full genome re-sequencing reveals a novel circadian clock mutation in Arabidopsis
    Ashelford, Kevin
    Eriksson, Maria E.
    Allen, Christopher M.
    D'Amore, Rosalinda
    Johansson, Mikael
    Gould, Peter
    Kay, Suzanne
    Millar, Andrew J.
    Hall, Neil
    Hall, Anthony
    GENOME BIOLOGY, 2011, 12 (03):
  • [26] Full genome re-sequencing reveals a novel circadian clock mutation in Arabidopsis
    Kevin Ashelford
    Maria E Eriksson
    Christopher M Allen
    Rosalinda D'Amore
    Mikael Johansson
    Peter Gould
    Suzanne Kay
    Andrew J Millar
    Neil Hall
    Anthony Hall
    Genome Biology, 12
  • [27] Direct regulation of abiotic responses by the Arabidopsis circadian clock component PRR7
    Liu, Tiffany
    Carlsson, Jenny
    Takeuchi, Tomomi
    Newton, Linsey
    Farre, Eva M.
    PLANT JOURNAL, 2013, 76 (01): : 101 - 114
  • [28] DET1 regulates the proteasomal degradation of LHY, a component of the Arabidopsis circadian clock
    Hae-Ryong Song
    Isabelle A. Carré
    Plant Molecular Biology, 2005, 57 : 761 - 771
  • [29] Direct regulation of abiotic responses by the Arabidopsis circadian clock component PRR7
    Liu, Tiffany
    Carlsson, Jenny
    Takeuchi, Tomomi
    Newton, Linsey
    Farré, Eva M.
    Plant Journal, 2013, 76 (01): : 101 - 114
  • [30] DET1 regulates the proteasomal degradation of LHY, a component of the Arabidopsis circadian clock
    Song, HR
    Carré, IA
    PLANT MOLECULAR BIOLOGY, 2005, 57 (05) : 761 - 771