A genome wide RNA interference screening method to identify host factors that modulate Influenza A virus replication

被引：6
|
作者：
Chin, Christopher R. [1 ]
Brass, Abraham L. [1 ]
机构：
[1] Univ Massachusetts, Sch Med, Microbiol & Physiol Syst Dept, Worcester, MA 01655 USA
来源：
METHODS | 2013年 / 59卷 / 02期
关键词：
siRNA screening; Automated siRNA transfection; Reverse transfection; Functional genomics; Host-virus interactions; Influenza A virus; Whole genome screen; WEST-NILE-VIRUS; MAMMALIAN-CELLS; GENETIC SCREEN; INFECTION;
D O I：
10.1016/j.ymeth.2012.09.009
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The use of genome wide RNA interference (RNAi) screens to investigate host-virals interactions has revealed unexpected connections that have improved our understanding of viral pathogenesis and cell biology. This work describes the use of an RNAi screening method employing an immunofluorescence image-based strategy and influenza A virus. We find this approach to be readily implemented, scalable and amenable to the direct evaluation of a variety of viral lifecycles. Published by Elsevier Inc.
引用
收藏
页码：217 / 224
页数：8
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