An antiviral protein having deoxyribonuclease and ribonuclease activity from leaves of the post-flowering stage of Celosia cristata

被引：17

作者：

Begam, M ^{[1
]}

Narwal, S ^{[1
]}

Roy, S ^{[1
]}

Kumar, S ^{[1
]}

Lodha, HL ^{[1
]}

Kapoor, HC ^{[1
]}

机构：

[1] Indian Agr Res Inst, Div Biochem, New Delhi 110012, India

来源：

BIOCHEMISTRY-MOSCOW | 2006年 / 71卷 / Suppl 1期

关键词：

Celosia cristata; antiviral proteins; ribosome inactivating protein; DNase activity; RNase activity;

D O I：

10.1134/S0006297906130074

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An antiviral protein named CCP-27 was purified from the leaves of Celosia cristata at the post-flowering stage by anion-exchange, cation-exchange, and get-filtration chromatography. It exhibited resistance against sunnhemp rosette virus in its test host Cyamopsis tetragonoloba. It also exhibited deoxyribonuclease activity against supercoiled pBlueScript SK+ plasmid DNA. It was found to nick supercoiled DNA into nicked circular form at lower protein concentration followed by nicked to linear form conversion at higher protein concentration. CCP-27 also possesses strong ribonuclease activity against Torula yeast rRNA.

引用

页码：S44 / S48

页数：5

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