False positive in the measurement of thyroglobulin induced by rheumatoid factor

被引：13
作者：
Astarita, G. [1 ]
Gutierrez, S. [1 ]
Kogovsek, N. [1 ]
Mormandi, E. [1 ]
Otero, P. [1 ]
Calabrese, C. [1 ]
Alcaraz, G. [1 ]
Vazquez, A. [1 ]
Abalovich, M. [1 ]
机构：
[1] Durand Hosp, Div Endocrinol, Buenos Aires, DF, Argentina
来源：
CLINICA CHIMICA ACTA | 2015年 / 447卷
关键词：
Thyroglobulin; Interference; Heterophilic antibodies; Rheumatoid factor; Differentiated thyroid cancer; ANTIBODY INTERFERENCE; SERUM THYROGLOBULIN; HETEROPHILE; ASSAYS;
D O I：
10.1016/j.cca.2015.04.039
中图分类号：
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号：
1001 ;
摘要：
Background: We report a case of interference in thyroglobulin (Tg) measurement in a woman with differentiated thyroid cancer and rheumatoid arthritis history. Due to discordant Tg in relation to TSH concentrations and negative images, we investigated possible interference in the measurement of Tg. Methods: During the follow-up we measured Tg by chemiluminescence (TgQL) using Immulite 2000 immunoassay system. To investigate possible interference in Tg measurement, we made serial dilutions, re-testing of Tg by an alternative method: electrochemiluminescence, Cobas 6000 analyzer (TgEQL), recovery test of Tg and polyethylene glycol (PEG) 6000 precipitation. Results: During the patient follow-up, the TgQL ranged between <0.3 and 16.1 ng/ml. In the evaluated serum samples very high titers of rheumatoid factor (RF) were found. When RF titers were lowered post PEG precipitation, Tg QL concentrations became undetectable. Conclusion: We describe an unusual case of interference in Tg assay due to RR When disagreement among Tg concentrations, images and clinical features is observed, we suggest taking into account the evaluation of possible Tg interference to avoid unnecessary complementary exams and inappropriate treatment. (C) 2015 Elsevier B.V. All rights reserved.
引用
收藏
页码：43 / 46
页数：4
相关论文
共 21 条
[1]  
Astarita G, 2012, BIOQUIM PATOL CLIN, V76, P13
[2]   Rheumatoid Factor and Its Interference with Cytokine Measurements: Problems and Solutions [J].
Bartels, Else Marie ;
Watjen, Inger Falbe ;
Andersen, Eva Littrup ;
Danneskiold-Samsoe, Bente ;
Bliddal, Henning ;
Ribel-Madsen, Soren .
ARTHRITIS, 2011,
[3]   Heterophilic antibody interference in immunometric assays [J].
Bolstad, Nils ;
Warren, David J. ;
Nustad, Kjell .
BEST PRACTICE & RESEARCH CLINICAL ENDOCRINOLOGY & METABOLISM, 2013, 27 (05) :647-661
[4]   Structure of human IgM rheumatoid factor Fab bound to its autoantigen IgG Fc reveals a novel topology of antibody-antigen interaction [J].
Corper, AL ;
Sohi, MK ;
Bonagura, VR ;
Steinitz, M ;
Jefferis, R ;
Feinstein, A ;
Beale, D ;
Taussig, MJ ;
Sutton, BJ .
NATURE STRUCTURAL BIOLOGY, 1997, 4 (05) :374-381
[5]   Improved multiplex immunoassay performance in human plasma and synovial fluid following removal of interfering heterophilic antibodies [J].
de Jager, W ;
Prakken, BJ ;
Bijlsma, JWJ ;
Kuis, W ;
Rijkers, GT .
JOURNAL OF IMMUNOLOGICAL METHODS, 2005, 300 (1-2) :124-135
[6]  
Després N, 1998, CLIN CHEM, V44, P440
[7]   Thyroxin overdose due to rheumatoid factor interferences in thyroid-stimulating hormone assays [J].
Georges, Agnes ;
Charrie, Anne ;
Raynaud, Sophie ;
Lombard, Christine ;
Corcuff, Jean-Benoit .
CLINICAL CHEMISTRY AND LABORATORY MEDICINE, 2011, 49 (05) :873-875
[8]   Evaluation of the BRAHMS Kryptor® Thyroglobulin Minirecovery Test in patients with differentiated thyroid carcinoma [J].
Giovanella, Luca ;
Imperiali, Mauro ;
Verburg, Frederik A. ;
Ceriani, Luca .
CLINICAL CHEMISTRY AND LABORATORY MEDICINE, 2013, 51 (02) :449-453
[9]   Heterophile antibodies may falsely increase or decrease thyroglobulin measurement in patients with differentiated thyroid carcinoma [J].
Giovanella, Luca ;
Keller, Franco ;
Ceriani, Luca ;
Tozzoli, Renato .
CLINICAL CHEMISTRY AND LABORATORY MEDICINE, 2009, 47 (08) :952-954
[10]   Towards a better understanding of heterophile (and the like) antibody interference with modem immunoassays [J].
Levinson, SS ;
Miller, JJ .
CLINICA CHIMICA ACTA, 2002, 325 (1-2) :1-15
123