Two-dimensional polyacrylamide gel electrophoresis (2D-PAGE): advances and perspectives

被引：150
|
作者：
Issaq, Haleem J. [1 ]
Veenstra, Timothy D. [1 ]
机构：
[1] NCI, SAIC Frederick Inc, Adv Technol Program, Lab Proteom & Analyt Technol, Frederick, MD 21702 USA
来源：
BIOTECHNIQUES | 2008年 / 44卷 / 05期
基金：
美国国家卫生研究院;
关键词：
D O I：
10.2144/000112823
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
The recent trend in science is to assay as many biological molecules as possible within a single experiment. This trend is evident in proteomics where the aim is to characterize thousands of proteins within cells, tissues, and organisms. While advances in mass spectrometry have been critical, developments made in two-dimensional PAGE (2D-PAGE) have also played a major role in enabling proteomics. In this review. we discuss and highlight the advances made in 2D-PAGE over the past 25 years that have made it a foundational tool in proteomic research.
引用
收藏
页码：697 / +
页数：3
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