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Investigating the candidacy of a lipoteichoic acid-based glycoconjugate as a vaccine to combat Clostridium difficile infection
被引:42
作者:

Cox, Andrew D.
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Natl Res Council Canada, Vaccine Program, Ottawa, ON K1A 0R6, Canada Natl Res Council Canada, Vaccine Program, Ottawa, ON K1A 0R6, Canada

St Michael, Frank
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Natl Res Council Canada, Vaccine Program, Ottawa, ON K1A 0R6, Canada Natl Res Council Canada, Vaccine Program, Ottawa, ON K1A 0R6, Canada

Aubry, Annie
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Natl Res Council Canada, Vaccine Program, Ottawa, ON K1A 0R6, Canada Natl Res Council Canada, Vaccine Program, Ottawa, ON K1A 0R6, Canada

Cairns, Chantelle M.
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Natl Res Council Canada, Vaccine Program, Ottawa, ON K1A 0R6, Canada Natl Res Council Canada, Vaccine Program, Ottawa, ON K1A 0R6, Canada

Strong, Philippa C. R.
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Natl Res Council Canada, Vaccine Program, Ottawa, ON K1A 0R6, Canada Natl Res Council Canada, Vaccine Program, Ottawa, ON K1A 0R6, Canada

Hayes, Alexander C.
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Natl Res Council Canada, Vaccine Program, Ottawa, ON K1A 0R6, Canada Natl Res Council Canada, Vaccine Program, Ottawa, ON K1A 0R6, Canada

Logan, Susan M.
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Natl Res Council Canada, Vaccine Program, Ottawa, ON K1A 0R6, Canada Natl Res Council Canada, Vaccine Program, Ottawa, ON K1A 0R6, Canada
机构:
[1] Natl Res Council Canada, Vaccine Program, Ottawa, ON K1A 0R6, Canada
关键词:
Clostridium difficile;
Conjugate vaccine;
Lipoteichoic acid;
MONOCLONAL-ANTIBODIES;
LIPOPOLYSACCHARIDE;
ANTIGENS;
D O I:
10.1007/s10719-013-9489-3
中图分类号:
Q5 [生物化学];
Q7 [分子生物学];
学科分类号:
071010 ;
081704 ;
摘要:
A lipoteichoic acid has recently been shown to be conserved in the majority of strains from Clostridium difficile and as such is being considered as a possible vaccine antigen. In this study we examine the candidacy of the conserved lipoteichoic acid by demonstrating that it is possible to elicit antibodies against C. difficile strains following immunisation of rabbits and mice with glycoconjugates elaborating the conserved lipoteichoic acid antigen. The present study describes a conjugation strategy that utilises an amino functionality, present at approximately 33 % substitution of the N-acetyl-glucosamine residues within the LTA polymer repeating unit, as the attachment point for conjugation. A maleimide-thiol linker strategy with the maleimide linker on the carboxyl residues of the carrier protein and the thiol linker on the carbohydrate was employed. Immunisation derived antisera from rabbits and mice, recognised all strains of C. difficile vegetative cells examined, despite an immune response to the linkers also being observed. These sera recognised live cells in an immunofluorescence assay and were also able to recognise the spore form of the bacterium. This study has illustrated that the LTA polymer is a highly conserved surface polymer of C. difficile that is easily accessible to the immune system and as such merits consideration as a vaccine antigen to combat C. difficile infection.
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页码:843 / 855
页数:13
相关论文
共 28 条
[1]
Phosphorylation of the Synthetic Hexasaccharide Repeating Unit Is Essential for the Induction of Antibodies to Clostridium difficile PSII Cell Wall Polysaccharide
[J].
Adamo, Roberto
;
Romano, Maria R.
;
Berti, Francesco
;
Leuzzi, Rosanna
;
Tontini, Marta
;
Danieli, Elisa
;
Cappelletti, Emilia
;
Cakici, Osman S.
;
Swennen, Erwin
;
Pinto, Vittoria
;
Brogioni, Barbara
;
Proietti, Daniela
;
Galeotti, Cesira L.
;
Lay, Luigi
;
Monteiro, Mario A.
;
Scarselli, Maria
;
Costantino, Paolo
.
ACS CHEMICAL BIOLOGY,
2012, 7 (08)
:1420-1428

Adamo, Roberto
论文数: 0 引用数: 0
h-index: 0
机构:
Novartis Vaccines & Diagnost, Res Ctr, I-53100 Siena, Italy Novartis Vaccines & Diagnost, Res Ctr, I-53100 Siena, Italy

Romano, Maria R.
论文数: 0 引用数: 0
h-index: 0
机构:
Novartis Vaccines & Diagnost, Res Ctr, I-53100 Siena, Italy Novartis Vaccines & Diagnost, Res Ctr, I-53100 Siena, Italy

Berti, Francesco
论文数: 0 引用数: 0
h-index: 0
机构:
Novartis Vaccines & Diagnost, Res Ctr, I-53100 Siena, Italy Novartis Vaccines & Diagnost, Res Ctr, I-53100 Siena, Italy

Leuzzi, Rosanna
论文数: 0 引用数: 0
h-index: 0
机构:
Novartis Vaccines & Diagnost, Res Ctr, I-53100 Siena, Italy Novartis Vaccines & Diagnost, Res Ctr, I-53100 Siena, Italy

Tontini, Marta
论文数: 0 引用数: 0
h-index: 0
机构:
Novartis Vaccines & Diagnost, Res Ctr, I-53100 Siena, Italy Novartis Vaccines & Diagnost, Res Ctr, I-53100 Siena, Italy

Danieli, Elisa
论文数: 0 引用数: 0
h-index: 0
机构:
Novartis Vaccines & Diagnost, Res Ctr, I-53100 Siena, Italy Novartis Vaccines & Diagnost, Res Ctr, I-53100 Siena, Italy

Cappelletti, Emilia
论文数: 0 引用数: 0
h-index: 0
机构:
Novartis Vaccines & Diagnost, Res Ctr, I-53100 Siena, Italy Novartis Vaccines & Diagnost, Res Ctr, I-53100 Siena, Italy

Cakici, Osman S.
论文数: 0 引用数: 0
h-index: 0
机构:
Novartis Vaccines & Diagnost, Res Ctr, I-53100 Siena, Italy Novartis Vaccines & Diagnost, Res Ctr, I-53100 Siena, Italy

Swennen, Erwin
论文数: 0 引用数: 0
h-index: 0
机构:
Novartis Vaccines & Diagnost, Res Ctr, I-53100 Siena, Italy Novartis Vaccines & Diagnost, Res Ctr, I-53100 Siena, Italy

Pinto, Vittoria
论文数: 0 引用数: 0
h-index: 0
机构:
Novartis Vaccines & Diagnost, Res Ctr, I-53100 Siena, Italy Novartis Vaccines & Diagnost, Res Ctr, I-53100 Siena, Italy

Brogioni, Barbara
论文数: 0 引用数: 0
h-index: 0
机构:
Novartis Vaccines & Diagnost, Res Ctr, I-53100 Siena, Italy Novartis Vaccines & Diagnost, Res Ctr, I-53100 Siena, Italy

Proietti, Daniela
论文数: 0 引用数: 0
h-index: 0
机构:
Novartis Vaccines & Diagnost, Res Ctr, I-53100 Siena, Italy Novartis Vaccines & Diagnost, Res Ctr, I-53100 Siena, Italy

Galeotti, Cesira L.
论文数: 0 引用数: 0
h-index: 0
机构:
Novartis Vaccines & Diagnost, Res Ctr, I-53100 Siena, Italy Novartis Vaccines & Diagnost, Res Ctr, I-53100 Siena, Italy

Lay, Luigi
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Milan, Dept Organ & Ind Chem, I-20133 Milan, Italy Novartis Vaccines & Diagnost, Res Ctr, I-53100 Siena, Italy

Monteiro, Mario A.
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Guelph, Dept Chem, Guelph, ON NIG 2W1, Canada Novartis Vaccines & Diagnost, Res Ctr, I-53100 Siena, Italy

Scarselli, Maria
论文数: 0 引用数: 0
h-index: 0
机构:
Novartis Vaccines & Diagnost, Res Ctr, I-53100 Siena, Italy Novartis Vaccines & Diagnost, Res Ctr, I-53100 Siena, Italy

Costantino, Paolo
论文数: 0 引用数: 0
h-index: 0
机构:
Novartis Vaccines & Diagnost, Res Ctr, I-53100 Siena, Italy Novartis Vaccines & Diagnost, Res Ctr, I-53100 Siena, Italy
[2]
Fecal Microbiota Transplantation for Recurrent Clostridium difficile Infection
[J].
Brandt, Lawrence J.
;
Reddy, Sheela S.
.
JOURNAL OF CLINICAL GASTROENTEROLOGY,
2011, 45
:S159-S167

Brandt, Lawrence J.
论文数: 0 引用数: 0
h-index: 0
机构:
Albert Einstein Coll Med, Montefiore Med Ctr, Moses Div, Bronx, NY 10467 USA Albert Einstein Coll Med, Montefiore Med Ctr, Moses Div, Bronx, NY 10467 USA

Reddy, Sheela S.
论文数: 0 引用数: 0
h-index: 0
机构:
Dept Med, Div Gastroenterol, Bronx, NY USA Albert Einstein Coll Med, Montefiore Med Ctr, Moses Div, Bronx, NY 10467 USA
[3]
PSEUDOMONAS EXOTOXIN CONTAINS A SPECIFIC SEQUENCE AT THE CARBOXYL TERMINUS THAT IS REQUIRED FOR CYTOTOXICITY
[J].
CHAUDHARY, VK
;
JINNO, Y
;
FITZGERALD, D
;
PASTAN, I
.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,
1990, 87 (01)
:308-312

CHAUDHARY, VK
论文数: 0 引用数: 0
h-index: 0

JINNO, Y
论文数: 0 引用数: 0
h-index: 0

FITZGERALD, D
论文数: 0 引用数: 0
h-index: 0

PASTAN, I
论文数: 0 引用数: 0
h-index: 0
[4]
Structural analysis of the lipopolysaccharide from Neisseria meningitidis strain BZ157 galE:: localisation of two phosphoethanolamine residues in the inner core oligosaccharide
[J].
Cox, AD
;
Li, JJ
;
Brisson, JR
;
Moxon, ER
;
Richards, JC
.
CARBOHYDRATE RESEARCH,
2002, 337 (16)
:1435-1444

Cox, AD
论文数: 0 引用数: 0
h-index: 0
机构: Natl Res Council Canada, Inst Biol Sci, Ottawa, ON K1A 0R6, Canada

Li, JJ
论文数: 0 引用数: 0
h-index: 0
机构: Natl Res Council Canada, Inst Biol Sci, Ottawa, ON K1A 0R6, Canada

Brisson, JR
论文数: 0 引用数: 0
h-index: 0
机构: Natl Res Council Canada, Inst Biol Sci, Ottawa, ON K1A 0R6, Canada

Moxon, ER
论文数: 0 引用数: 0
h-index: 0
机构: Natl Res Council Canada, Inst Biol Sci, Ottawa, ON K1A 0R6, Canada

Richards, JC
论文数: 0 引用数: 0
h-index: 0
机构: Natl Res Council Canada, Inst Biol Sci, Ottawa, ON K1A 0R6, Canada
[5]
Investigating the potential of conserved inner core oligosaccharide regions of Moraxella catarrhalis lipopolysaccharide as vaccine antigens: accessibility and functional activity of monoclonal antibodies and glycoconjugate derived sera
[J].
Cox, Andrew D.
;
Michael, Frank St.
;
Cairns, Chantelle M.
;
Lacelle, Suzanne
;
Filion, Amy Lea
;
Neelamegan, Dhamodharan
;
Wenzel, Cory Q.
;
Horan, Heather
;
Richards, James C.
.
GLYCOCONJUGATE JOURNAL,
2011, 28 (3-4)
:165-182

Cox, Andrew D.
论文数: 0 引用数: 0
h-index: 0
机构:
Natl Res Council Canada, Inst Biol Sci, Ottawa, ON K1A 0R6, Canada Natl Res Council Canada, Inst Biol Sci, Ottawa, ON K1A 0R6, Canada

Michael, Frank St.
论文数: 0 引用数: 0
h-index: 0
机构:
Natl Res Council Canada, Inst Biol Sci, Ottawa, ON K1A 0R6, Canada Natl Res Council Canada, Inst Biol Sci, Ottawa, ON K1A 0R6, Canada

Cairns, Chantelle M.
论文数: 0 引用数: 0
h-index: 0
机构:
Natl Res Council Canada, Inst Biol Sci, Ottawa, ON K1A 0R6, Canada Natl Res Council Canada, Inst Biol Sci, Ottawa, ON K1A 0R6, Canada

Lacelle, Suzanne
论文数: 0 引用数: 0
h-index: 0
机构:
Natl Res Council Canada, Inst Biol Sci, Ottawa, ON K1A 0R6, Canada Natl Res Council Canada, Inst Biol Sci, Ottawa, ON K1A 0R6, Canada

Filion, Amy Lea
论文数: 0 引用数: 0
h-index: 0
机构:
Natl Res Council Canada, Inst Biol Sci, Ottawa, ON K1A 0R6, Canada Natl Res Council Canada, Inst Biol Sci, Ottawa, ON K1A 0R6, Canada

Neelamegan, Dhamodharan
论文数: 0 引用数: 0
h-index: 0
机构:
Natl Res Council Canada, Inst Biol Sci, Ottawa, ON K1A 0R6, Canada Natl Res Council Canada, Inst Biol Sci, Ottawa, ON K1A 0R6, Canada

Wenzel, Cory Q.
论文数: 0 引用数: 0
h-index: 0
机构:
Natl Res Council Canada, Inst Biol Sci, Ottawa, ON K1A 0R6, Canada Natl Res Council Canada, Inst Biol Sci, Ottawa, ON K1A 0R6, Canada

Horan, Heather
论文数: 0 引用数: 0
h-index: 0
机构:
Natl Res Council Canada, Inst Biol Sci, Ottawa, ON K1A 0R6, Canada Natl Res Council Canada, Inst Biol Sci, Ottawa, ON K1A 0R6, Canada

Richards, James C.
论文数: 0 引用数: 0
h-index: 0
机构:
Natl Res Council Canada, Inst Biol Sci, Ottawa, ON K1A 0R6, Canada Natl Res Council Canada, Inst Biol Sci, Ottawa, ON K1A 0R6, Canada
[6]
Investigating the candidacy of LPS-based glycoconjugates to prevent invasive meningococcal disease: immunology of glycoconjugates with high carbohydrate loading
[J].
Cox, Andrew D.
;
St Michael, Frank
;
Neelamegan, Dhamodharan
;
Lacelle, Suzanne
;
Cairns, Chantelle M.
;
Giuliani, Marzia M.
;
Biolchi, Alessia
;
Hoe, J. Claire
;
Moxon, E. Richard
;
Richards, James C.
.
GLYCOCONJUGATE JOURNAL,
2010, 27 (7-9)
:643-648

Cox, Andrew D.
论文数: 0 引用数: 0
h-index: 0
机构:
CNR, Inst Biol Sci, Ottawa, ON K1A 0R6, Canada CNR, Inst Biol Sci, Ottawa, ON K1A 0R6, Canada

St Michael, Frank
论文数: 0 引用数: 0
h-index: 0
机构:
CNR, Inst Biol Sci, Ottawa, ON K1A 0R6, Canada CNR, Inst Biol Sci, Ottawa, ON K1A 0R6, Canada

Neelamegan, Dhamodharan
论文数: 0 引用数: 0
h-index: 0
机构:
CNR, Inst Biol Sci, Ottawa, ON K1A 0R6, Canada CNR, Inst Biol Sci, Ottawa, ON K1A 0R6, Canada

Lacelle, Suzanne
论文数: 0 引用数: 0
h-index: 0
机构:
CNR, Inst Biol Sci, Ottawa, ON K1A 0R6, Canada CNR, Inst Biol Sci, Ottawa, ON K1A 0R6, Canada

Cairns, Chantelle M.
论文数: 0 引用数: 0
h-index: 0
机构:
CNR, Inst Biol Sci, Ottawa, ON K1A 0R6, Canada CNR, Inst Biol Sci, Ottawa, ON K1A 0R6, Canada

Giuliani, Marzia M.
论文数: 0 引用数: 0
h-index: 0
机构:
Novartis Vaccines, I-53100 Siena, Italy CNR, Inst Biol Sci, Ottawa, ON K1A 0R6, Canada

Biolchi, Alessia
论文数: 0 引用数: 0
h-index: 0
机构:
Novartis Vaccines, I-53100 Siena, Italy CNR, Inst Biol Sci, Ottawa, ON K1A 0R6, Canada

Hoe, J. Claire
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Oxford, Weatherall Inst Mol Med, Headington OX3 9DU, Oxon, England CNR, Inst Biol Sci, Ottawa, ON K1A 0R6, Canada

Moxon, E. Richard
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Oxford, Weatherall Inst Mol Med, Headington OX3 9DU, Oxon, England CNR, Inst Biol Sci, Ottawa, ON K1A 0R6, Canada

Richards, James C.
论文数: 0 引用数: 0
h-index: 0
机构:
CNR, Inst Biol Sci, Ottawa, ON K1A 0R6, Canada CNR, Inst Biol Sci, Ottawa, ON K1A 0R6, Canada
[7]
First Synthesis of C. difficile PS-II Cell Wall Polysaccharide Repeating Unit
[J].
Danieli, Elise
;
Lay, Luigi
;
Proietti, Daniela
;
Berti, Francesco
;
Costantino, Paolo
;
Adamo, Roberto
.
ORGANIC LETTERS,
2011, 13 (03)
:378-381

Danieli, Elise
论文数: 0 引用数: 0
h-index: 0
机构:
Novartis Vaccines & Diagnost, Vaccine Chem Dept, I-53100 Siena, Italy Novartis Vaccines & Diagnost, Vaccine Chem Dept, I-53100 Siena, Italy

Lay, Luigi
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Milan, Dept Organ & Ind Chem, CISI, I-20133 Milan, Italy
Univ Milan, ISTM CNR, I-20133 Milan, Italy Novartis Vaccines & Diagnost, Vaccine Chem Dept, I-53100 Siena, Italy

Proietti, Daniela
论文数: 0 引用数: 0
h-index: 0
机构:
Novartis Vaccines & Diagnost, Vaccine Chem Dept, I-53100 Siena, Italy Novartis Vaccines & Diagnost, Vaccine Chem Dept, I-53100 Siena, Italy

Berti, Francesco
论文数: 0 引用数: 0
h-index: 0
机构:
Novartis Vaccines & Diagnost, Vaccine Chem Dept, I-53100 Siena, Italy Novartis Vaccines & Diagnost, Vaccine Chem Dept, I-53100 Siena, Italy

Costantino, Paolo
论文数: 0 引用数: 0
h-index: 0
机构:
Novartis Vaccines & Diagnost, Vaccine Chem Dept, I-53100 Siena, Italy Novartis Vaccines & Diagnost, Vaccine Chem Dept, I-53100 Siena, Italy

Adamo, Roberto
论文数: 0 引用数: 0
h-index: 0
机构:
Novartis Vaccines & Diagnost, Vaccine Chem Dept, I-53100 Siena, Italy Novartis Vaccines & Diagnost, Vaccine Chem Dept, I-53100 Siena, Italy
[8]
Clostridium difficile cell-surface polysaccharides composed of pentaglycosyl and hexaglycosyl phosphate repeating units
[J].
Ganeshapillai, Jeyabarathy
;
Vinogradov, Evguenii
;
Rousseau, Joyce
;
Weese, J. Scott
;
Monteiro, Mario A.
.
CARBOHYDRATE RESEARCH,
2008, 343 (04)
:703-710

Ganeshapillai, Jeyabarathy
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Guelph, Dept Chem, Guelph, ON N1G 2W1, Canada Univ Guelph, Dept Chem, Guelph, ON N1G 2W1, Canada

Vinogradov, Evguenii
论文数: 0 引用数: 0
h-index: 0
机构:
Natl Res Council Canada, Ottawa, ON K1A 0R6, Canada Univ Guelph, Dept Chem, Guelph, ON N1G 2W1, Canada

Rousseau, Joyce
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Guelph, Dept Pathobiol, Guelph, ON N1G 2W1, Canada Univ Guelph, Dept Chem, Guelph, ON N1G 2W1, Canada

Weese, J. Scott
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Guelph, Dept Pathobiol, Guelph, ON N1G 2W1, Canada Univ Guelph, Dept Chem, Guelph, ON N1G 2W1, Canada

Monteiro, Mario A.
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Guelph, Dept Chem, Guelph, ON N1G 2W1, Canada Univ Guelph, Dept Chem, Guelph, ON N1G 2W1, Canada
[9]
Meningococcal glycoconjugate vaccines
[J].
Gasparini, Roberto
;
Panatto, Donatella
.
HUMAN VACCINES,
2011, 7 (02)
:170-182

Gasparini, Roberto
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Genoa, Dept Hlth Sci, Genoa, Italy Univ Genoa, Dept Hlth Sci, Genoa, Italy

Panatto, Donatella
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Genoa, Dept Hlth Sci, Genoa, Italy Univ Genoa, Dept Hlth Sci, Genoa, Italy
[10]
Measures to control and prevent Clostridium difficile infection
[J].
Gerding, Dale N.
;
Muto, Carlene A.
;
Owens, Robert C., Jr.
.
CLINICAL INFECTIOUS DISEASES,
2008, 46
:S43-S49

Gerding, Dale N.
论文数: 0 引用数: 0
h-index: 0
机构:
US Dept Vet Affairs, Vet Affairs Edward Hines Jr Hosp, Hines, IL 60141 USA
Loyola Univ, Stritch Sch Med, Chicago, IL 60611 USA US Dept Vet Affairs, Vet Affairs Edward Hines Jr Hosp, Hines, IL 60141 USA

Muto, Carlene A.
论文数: 0 引用数: 0
h-index: 0
机构:
Univ Pittsburgh, Med Ctr, Pittsburgh, PA USA US Dept Vet Affairs, Vet Affairs Edward Hines Jr Hosp, Hines, IL 60141 USA

Owens, Robert C., Jr.
论文数: 0 引用数: 0
h-index: 0
机构:
Maine Med Ctr, Portland, ME 04102 USA
Univ Vermont, Coll Med, Burlington, VT USA US Dept Vet Affairs, Vet Affairs Edward Hines Jr Hosp, Hines, IL 60141 USA