Metallothionein dimers studied by nano-spray mass spectrometry

被引：25

作者：

Hathout, Y ^{[1
]}

Reynolds, KJ ^{[1
]}

Szilagyi, Z ^{[1
]}

Fenselau, C ^{[1
]}

机构：

[1] Univ Maryland, Dept Chem & Biochem, College Pk, MD 20742 USA

来源：

JOURNAL OF INORGANIC BIOCHEMISTRY | 2002年 / 88卷 / 02期

关键词：

metallothionein; dimer; electrospray mass spectrometry; nanospray; noncovalent complexes; metalloprotein;

D O I：

10.1016/S0162-0134(01)00375-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Both transient and stable dimers of metallothionein have been characterized, based on earlier studies using NMR, circular dichroism and size-exclusion chromatography. Here additional characterization is provided by nanospray mass spectrometry. Rapid redistribution of metal ions between monomeric Cd-7- and Zn-7-metallothionein 2a is monitored by nanospray. An experiment in which theses two forms of the monomeric protein are separated by a dialysis membrane, which will pass metal ions but not proteins, confirms that a transient dimer must form for metal ions to be redistributed. On the other hand, size-exclusion chromatography of reconstituted Zn-7- or Cd-7-metallothionein revealed the presence of monomeric and dimeric species. These dimers do not equilibrate readily to form monomers and they are shown to be covalent. (C) 2002 Elsevier Science B V All rights reserved.

引用

页码：119 / 122

页数：4

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