Tempol (4-hydroxy tempo) protects mice from cisplatin-induced acute kidney injuryviamodulation of expression of aquaporins and kidney injury molecule-1

被引：9

作者：

Afjal, Mohd Amir ^{[1
]}

Goswami, Poonam ^{[1
]}

Ahmad, Shahzad ^{[1
]}

Dabeer, Sadaf ^{[1
]}

Akhter, Juheb ^{[1
]}

Salman, Mohd ^{[2
]}

Mangla, Anuradha ^{[1
]}

Raisuddin, Sheikh ^{[1
]}

机构：

[1] Jamia Hamdard, Dept Med Elementol & Toxicol, Mol Toxicol Lab, New Delhi, India

[2] Jamia Hamdard, Dept Med Elementol & Toxicol, Mol Neurobiol Lab, New Delhi, India

来源：

DRUG AND CHEMICAL TOXICOLOGY | 2022年 / 45卷 / 03期

关键词：

Anti-neoplastic drug; acute kidney injury; electrolyte imbalance; nephric reabsorption; nephroprotection; ENDOPLASMIC-RETICULUM STRESS; INDUCED NEPHROTOXICITY; OXIDATIVE STRESS; WATER CHANNELS; RAT MODEL; VASOPRESSIN; MECHANISMS; BIOMARKERS; DECREASES; ALPHA;

D O I：

10.1080/01480545.2020.1831011

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Tempol (4-hydroxy tempo), a pleiotropic antioxidant is reported to afford protection against cisplatin (CP)-induced nephrotoxicity. However, molecular mechanisms of action of tempol in improving the renal function in CP-induced nephrotoxicity are not fully understood. We investigated the attenuating effect of tempol against CP-induced alterations in kidney injury molecule-1 (KIM-1) and aquaporins (AQPs) in mice. Tempol (100 mg/kg,po) pretreatment with CP (20 mg/kgip) showed restoration in renal function markers including electrolytes. CP treatment upregulated mRNA expression of KIM-1 and downregulated AQP and arginine vasopressin (AVP) expression which was attenuated by tempol. Immunoblotting analysis revealed that CP-induced alterations in KIM-1 and AQP expression were restored by tempol. Immunofluorocense study also showed restorative effect of tempol on the expression of AQP2 in CP-treated mice. In conclusion, this study provides experimental evidence that tempol resolved urinary concentration defect by the restoration of AQP, AVP and KIM-1 levels indicating a potential use of tempol in ameliorating the AKI in cancer patients under the treatment with CP.

引用

页码：1355 / 1363

页数：9

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