Polyvinyl alcohol-silica nanohybrids: An efficient carrier matrix for amylase immobilization

Polyvinyl alcohol (PVA)-silica nanohybrids have been synthesized in a modified Stober process. The bioactivities of the enzyme loaded hybrids were monitored and the optimum activity sample (H) was calcined at 300 degrees C in N-2 to obtain hybrid gel (H-3) with improved performance. The synthesized hybrids have been characterized by Fourier Transform Infra Red spectroscopy, X-ray diffraction, scanning electron microscopy, thermogravimetric analysis and BET surface area analysis. Under the optimized conditions, the bioactivity of the enzyme impregnated H-3 (H-3-Enz) was 21.823 U/mg. On recycling, H-3-Enz retained 88% of its initial bioactivity in the sixth cycle. The kinetic parameters of soluble starch hydrolysis for the immobilized (K-M = 4.137 mg mL(-1); V-max = 5.95 mg mL(-1) min(-1)) and free enzyme (K-M = 10.667 mg mL(-1); V-max = 6.0557 mg mL(-1) min(-1)) indicated that the immobilization has nearly doubled the enzyme's affinity for the substrate, while the maximum rate of the enzymatic reaction at the saturation point was not much affected. The immobilized enzyme showed greater shelf life in comparison to the free enzyme. (c) 2012 Elsevier Ltd. All rights reserved.