A Novel Mass Spectrometry-Based Assay for the Accurate Measurement of Thyroglobulin From Patient Samples Containing Antithyroglobulin Autoantibodies

被引:60
作者
Clarke, Nigel J. [1 ]
Zhang, Yanni [1 ]
Reitz, Richard E. [1 ]
机构
[1] Quest Diagnost Nichols Inst, Dept Endocrinol, San Juan Capistrano, CA 92675 USA
关键词
thyroid cancer; thyroglobulin; mass spectrometry;
D O I
10.2310/JIM.0b013e318276deb4
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Thyroid cancer is the most common endocrine cancer in the United States. The primary treatment of thyroid cancer is partial or complete thyroidectomy in patients in whom the cancer is discovered preoperatively, and is often followed by radioactive iodine ablation. After the initial therapy, patients are followed up regularly, most commonly by measurement of serum thyroglobulin (Tg) levels and high-resolution neck ultrasound. As Tg is only produced within the thyroid gland, it has long been recognized as an excellent biomarker for the presence of residual disease after treatment. A number of immunoassays are in common use to measure serum Tg levels. Unfortunately, irrespective of which method is chosen, there is a major potential artifact, in that the presence of autoantibodies binding to Tg (ATG) may bias the results to the point where they are clinically unreliable. This article describes a liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for the measurement of serum Tg after tryptic digestion of serum samples. The method is compared to Tg analyses using both a Food and Drug Administration-approved immunometric assay (IMA) and a well-respected, clinically used radioimmunoassay. In the absence of ATG, the new LC-MS/MS assay demonstrates equivalency compared to the IMA. However, in ATG-positive patients, the IMA Tg results are lower than the LC-MS/MS assay and the radioimmunoassay Tg results are typically higher. These studies demonstrate the accuracy and validity of the measurement of Tg by LC-MS/MS. This assay will permit the accurate determination of Tg levels even in patients with ATG.
引用
收藏
页码:1157 / 1163
页数:7
相关论文
共 11 条
[1]   Preface Mass Spectrometry Continues Its March into the Clinical Laboratory [J].
Clarke, Nigel J. ;
Hoofnagle, Andrew N. .
CLINICS IN LABORATORY MEDICINE, 2011, 31 (03) :IX-XI
[2]   Revised American Thyroid Association Management Guidelines for Patients with Thyroid Nodules and Differentiated Thyroid Cancer [J].
Cooper, David S. ;
Doherty, Gerard M. ;
Haugen, Bryan R. ;
Kloos, Richard T. ;
Lee, Stephanie L. ;
Mandel, Susan J. ;
Mazzaferri, Ernest L. ;
McIver, Bryan ;
Pacini, Furio ;
Schlumberger, Martin ;
Sherman, Steven I. ;
Steward, David L. ;
Tuttle, R. Michael .
THYROID, 2009, 19 (11) :1167-1214
[3]   Associations of serum thyrotropin concentrations with recurrence and death in differentiated thyroid cancer [J].
Hovens, Guido C. ;
Stokkel, Marcel P. ;
Kievit, Job ;
Corssmit, Eleonora P. ;
Pereira, Alberto M. ;
Romijn, Johannes A. ;
Smit, Johannes W. A. .
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 2007, 92 (07) :2610-2615
[4]   Outcomes of patients with differentiated thyroid carcinoma following initial therapy [J].
Jonklaas, Jacqueline ;
Sarlis, Nicholas J. ;
Litofsky, Danielle ;
Ain, Kenneth B. ;
Bigos, S. Thomas ;
Brierley, James D. ;
Cooper, David S. ;
Haugen, Bryan R. ;
Ladenson, Paul W. ;
Magner, James ;
Robbins, Jacob ;
Ross, Douglas S. ;
Skarulis, Monica ;
Maxon, Harry R. ;
Sherman, Steven I. .
THYROID, 2006, 16 (12) :1229-1242
[5]  
Latrofa F, 2012, J CLIN ENDOCR METAB, P97
[6]  
Nedelkov D, 2012, EXPERT REV MOL DIAGN, V12, P235, DOI [10.1586/ERM.12.15, 10.1586/erm.12.15]
[7]   Approach to and Treatment of Differentiated Thyroid Carcinoma [J].
Pacini, Furio ;
Castagna, Maria Grazia .
MEDICAL CLINICS OF NORTH AMERICA, 2012, 96 (02) :369-+
[8]   Strategies of Radioiodine Ablation in Patients with Low-Risk Thyroid Cancer [J].
Schlumberger, Martin ;
Catargi, Bogdan ;
Borget, Isabelle ;
Deandreis, Desiree ;
Zerdoud, Slimane ;
Bridji, Boumediene ;
Bardet, Stephane ;
Leenhardt, Laurence ;
Bastie, Delphine ;
Schvartz, Claire ;
Vera, Pierre ;
Morel, Olivier ;
Benisvy, Danielle ;
Bournaud, Claire ;
Bonichon, Francoise ;
Dejax, Catherine ;
Toubert, Marie-Elisabeth ;
Leboulleux, Sophie ;
Ricard, Marcel ;
Benhamou, Ellen .
NEW ENGLAND JOURNAL OF MEDICINE, 2012, 366 (18) :1663-1673
[9]   Cancer treatment and survivorship statistics, 2012 [J].
Siegel, Rebecca ;
DeSantis, Carol ;
Virgo, Katherine ;
Stein, Kevin ;
Mariotto, Angela ;
Smith, Tenbroeck ;
Cooper, Dexter ;
Gansler, Ted ;
Lerro, Catherine ;
Fedewa, Stacey ;
Lin, Chunchieh ;
Leach, Corinne ;
Cannady, Rachel Spillers ;
Cho, Hyunsoon ;
Scoppa, Steve ;
Hachey, Mark ;
Kirch, Rebecca ;
Jemal, Ahmedin ;
Ward, Elizabeth .
CA-A CANCER JOURNAL FOR CLINICIANS, 2012, 62 (04) :220-241
[10]   Serum thyroglobulin autoantibodies: Prevalence, influence on serum thyroglobulin measurement, and prognostic significance in patients with differentiated thyroid carcinoma [J].
Spencer, CA ;
Takeuchi, M ;
Kazarosyan, M ;
Wang, CC ;
Guttler, RB ;
Singer, PA ;
Fatemi, S ;
LoPresti, JS ;
Nicoloff, JT .
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM, 1998, 83 (04) :1121-1127