Crystallization and preliminary X-ray diffraction data of an archaeal asparagine synthetase related to asparaginyl-tRNA synthetase

The archaebacterial-type asparagine synthetase A from Pyrococcus abyssi (AS-AR), which is related to asparaginyl-tRNA synthetase, was crystallized in two different conditions using the hanging-drop vapour-diffusion method. Crystals belonging to space group C2 with unit-cell parameters a=103.6, b=43.3, c=121.5 Angstrom, beta=112.6degrees and one dimer per asymmetric unit were obtained in the presence of 2-propanol and PEG 4000 at pH 5.6. Another crystal form was obtained in the presence of dioxan and belongs to the monoclinic space group P2(1), with unit-cell parameters a=96.8, b=103.9, c=98.4 Angstrom, beta=107.5degrees and two dimers per asymmetric unit. Two different native diffraction data sets were collected to 2.3 and 3.0 Angstrom resolution using synchrotron radiation and cryocooling for crystals belonging to space groups C2 and P2(1), respectively.