Ancestral Absence of Electron Transport Chains in Patescibacteria and DPANN

被引：56

作者：

Beam, Jacob P. ^{[1
]}

Becraft, Eric D. ^{[1
]}

Brown, Julia M. ^{[1
]}

Schulz, Frederik ^{[2
]}

Jarett, Jessica K. ^{[2
]}

Bezuidt, Oliver ^{[1
]}

Poulton, Nicole J. ^{[1
]}

Clark, Kayla ^{[1
]}

Dunfield, Peter F. ^{[3
]}

Ravin, Nikolai V. ^{[4
]}

Spear, John R. ^{[5
]}

Hedlund, Brian P. ^{[6
]}

Kormas, Konstantinos A. ^{[7
]}

Sievert, Stefan M. ^{[8
]}

Elshahed, Mostafa S. ^{[9
]}

Barton, Hazel A. ^{[10
]}

Stott, Matthew B. ^{[11
]}

Eisen, Jonathan A. ^{[12
]}

Moser, Duane P. ^{[13
]}

Onstott, Tullis C. ^{[14
]}

Woyke, Tanja ^{[2
]}

Stepanauskas, Ramunas ^{[1
]}

机构：

[1] Bigelow Lab Ocean Sci, East Boothbay, ME 04544 USA

[2] Joint Genome Inst, Dept Energy, Berkeley, CA USA

[3] Univ Calgary, Dept Biol Sci, Calgary, AB, Canada

[4] Russian Acad Sci, Res Ctr Biotechnol, Inst Bioengn, Moscow, Russia

[5] Colorado Sch Mines, Civil & Environm Engn, Golden, CO 80401 USA

[6] Univ Nevada, Nevada Inst Personalized Med, Sch Life Sci, Las Vegas, NV 89154 USA

[7] Univ Thessaly, Dept Ichthyol & Aquat Environm, Volos, Greece

[8] Woods Hole Oceanog Inst, Dept Biol, Woods Hole, MA 02543 USA

[9] Oklahoma State Univ, Dept Microbiol & Mol Genet, Stillwater, OK 74078 USA

[10] Univ Akron, Dept Biol, Akron, OH 44325 USA

[11] Univ Canterbury, Sch Biol Sci, Christchurch, New Zealand

[12] Univ Calif Davis, Genome Ctr, Dept Med Microbiol & Immunol, Dept Ecol & Evolut, Davis, CA 95616 USA

[13] Desert Res Inst, Las Vegas, NV USA

[14] Princeton Univ, Dept Geosci, Princeton, NJ 08544 USA

来源：

FRONTIERS IN MICROBIOLOGY | 2020年 / 11卷

基金：

美国国家科学基金会; 俄罗斯科学基金会;

关键词：

Bacteria; Archaea; evolution; genomics fermentation; respiration; oxidoreductases; CANDIDATE PHYLUM; GENOME; BACTERIA; EVOLUTION; INSIGHTS; TREE; CULTIVATION; HOST; GENE;

D O I：

10.3389/fmicb.2020.01848

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Recent discoveries suggest that the candidate superphyla Patescibacteria and DPANN constitute a large fraction of the phylogenetic diversity of Bacteria and Archaea. Their small genomes and limited coding potential have been hypothesized to be ancestral adaptations to obligate symbiotic lifestyles. To test this hypothesis, we performed cell-cell association, genomic, and phylogenetic analyses on 4,829 individual cells of Bacteria and Archaea from 46 globally distributed surface and subsurface field samples. This confirmed the ubiquity and abundance of Patescibacteria and DPANN in subsurface environments, the small size of their genomes and cells, and the divergence of their gene content from other Bacteria and Archaea. Our analyses suggest that most Patescibacteria and DPANN in the studied subsurface environments do not form specific physical associations with other microorganisms. These data also suggest that their unusual genomic features and prevalent auxotrophies may be a result of ancestral, minimal cellular energy transduction mechanisms that lack respiration, thus relying solely on fermentation for energy conservation.

引用

页数：16

共 89 条

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