The effect of dental restorative materials on dental biofilm

被引:125
作者
Auschill, TM
Arweiler, NB
Brecx, M
Reich, E
Sculean, A
Netuschil, L
机构
[1] Univ Freiburg, Dept Operat Dent & Periodontol, D-79106 Freiburg, Germany
[2] Dresden Univ Technol, Med Fac Carl Gustav Carus, Dept Conservat Dent, D-8027 Dresden, Germany
[3] Univ Saarland, Dept Periodontol & Conservat Dent, D-6650 Homburg, Germany
关键词
dental biofilm; biofilm vitality; confocal laser scanning microscope (CLSM); dental materials;
D O I
10.1046/j.0909-8836.2001.101160.x
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
To investigate the arrangement of biofilms formed in vivo, volunteers wore splints with slabs of six different dental materials inserted to collect smooth surface plaque. After 5 d of undisturbed plaque accumulation, the specimens were vital stained and analyzed by the confocal laser scanning microscopy (CLSM) to evaluate the percentage of vital biofilm microflora (VF percentage). Further parameters were the area of the specimens covered by plaque (surface coatings SC, %) and the height of the biofilms (BH, mum). The metals amalgam and gold, the compomer, as well as the glass-ionomer cement harboured an almost entirely dead biofilm (VF <8%). Resin composite led to vitality values between 4 and 21%, while a very thin biofilm on ceramic revealed the highest vitality values (34 86%). SC varied from 6% on glass-ionomer cement to 100% on amalgam. BH reached its highest value on amalgam and gold of 17 and 11 mum, respectively, while heights of between 1 and 6 mum were found on the ceramic, resin composite, compomer and the glass-ionomer cement. Within their limits, the present findings indicate that amalgam, gold, compomer and glass-ionomer cement exert an influence against the adhering biofilm. No general relationship could be established between the different parameters VF percentage, SC percentage and BH (mum).
引用
收藏
页码:48 / 53
页数:6
相关论文
共 55 条
  • [1] Alcohol-free mouthrinse solutions to reduce supragingival plaque regrowth and vitality - A controlled clinical study
    Arweiler, NB
    Netuschil, L
    Reich, E
    [J]. JOURNAL OF CLINICAL PERIODONTOLOGY, 2001, 28 (02) : 168 - 174
  • [2] AUGTHUN M, 1988, DEUT ZAHNAERZTL Z, V43, P869
  • [3] Spatial distribution of vital and dead microorganisms in dental biofilms
    Auschill, TM
    Artweiler, NB
    Netuschil, L
    Brecx, M
    Reich, E
    Sculean, A
    [J]. ARCHIVES OF ORAL BIOLOGY, 2001, 46 (05) : 471 - 476
  • [4] EFFICACY OF LISTERINE, MERIDOL AND CHLORHEXIDINE MOUTHRINSES ON PLAQUE, GINGIVITIS AND PLAQUE BACTERIA VITALITY
    BRECX, M
    NETUSCHIL, L
    REICHERT, B
    SCHREIL, G
    [J]. JOURNAL OF CLINICAL PERIODONTOLOGY, 1990, 17 (05) : 292 - 297
  • [5] Brecx M, 1994, J West Soc Periodontol Periodontal Abstr, V42, P77
  • [6] EARLY FORMATION OF DENTAL PLAQUE ON PLASTIC FILMS .2. ELECTRON-MICROSCOPIC OBSERVATIONS
    BRECX, M
    RONSTROM, A
    THEILADE, J
    ATTSTROM, R
    [J]. JOURNAL OF PERIODONTAL RESEARCH, 1981, 16 (02) : 213 - 227
  • [7] PLAQUE RETENTION ON TEETH RESTORED WITH FULL-CERAMIC CROWNS - A COMPARATIVE-STUDY
    CHAN, C
    WEBER, H
    [J]. JOURNAL OF PROSTHETIC DENTISTRY, 1986, 56 (06) : 666 - 671
  • [8] Bacterial biofilms: A common cause of persistent infections
    Costerton, JW
    Stewart, PS
    Greenberg, EP
    [J]. SCIENCE, 1999, 284 (5418) : 1318 - 1322
  • [9] MICROBIAL BIOFILMS
    COSTERTON, JW
    LEWANDOWSKI, Z
    CALDWELL, DE
    KORBER, DR
    LAPPINSCOTT, HM
    [J]. ANNUAL REVIEW OF MICROBIOLOGY, 1995, 49 : 711 - 745
  • [10] THE BACTERIAL GLYCOCALYX IN NATURE AND DISEASE
    COSTERTON, JW
    IRVIN, RT
    CHENG, KJ
    [J]. ANNUAL REVIEW OF MICROBIOLOGY, 1981, 35 : 299 - 324