Probing transient non-native states in amyloid beta fiber elongation by NMR

被引：46

作者：

Brender, Jeffrey R. ^{[1
,2
]}

Ghosh, Anirban ^{[3
]}

Kotler, Samuel A. ^{[2
]}

Krishnamoorthy, Janarthanan ^{[1
,2
,4
]}

Bera, Swapna ^{[3
]}

Morris, Vanessa ^{[5
]}

Sil, Timir Baran ^{[6
]}

Garai, Kanchan ^{[6
]}

Reif, Bernd ^{[5
]}

Bhunia, Anirban ^{[1
,2
,3
]}

Ramamoorthy, Ayyalusamy ^{[1
,2
,7
]}

机构：

[1] Univ Michigan, Biophys, Ann Arbor, MI 48109 USA

[2] Univ Michigan, Dept Chem, Ann Arbor, MI 48109 USA

[3] Bose Inst, Dept Biophys, Kolkata 700054, India

[4] VClinbio Labs Pvt Ltd, Sri Ramchandra Med Ctr, Chennai 600116, Tamil Nadu, India

[5] Tech Univ Munich, Dept Chem, Munich, Germany

[6] TIFR Ctr Interdisciplinary Sci, Hyderabad 500107, Telangana, India

[7] Tech Univ Munich, Inst Adv Studies, Munich, Germany

来源：

CHEMICAL COMMUNICATIONS | 2019年 / 55卷 / 31期

关键词：

SECONDARY NUCLEATION; AGGREGATION; PROTOFIBRILS; DYNAMICS; RESOLUTION; MECHANISM; MONOMER; REVEALS;

D O I：

10.1039/c9cc01067j

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Using NMR to probe transient binding of A(1-40) monomers to fibers, we find partially bound conformations with the highest degree of interaction near F19-K28 and a lesser degree of interaction near the C-terminus (L34-G37). This represents a shift away from the KLVFFA recognition sequence (residues 16-21) currently used for inhibitor design.

引用

页码：4483 / 4486

页数：4

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