Determination of polycyclic aromatic hydrocarbon content in heat-treated meat retailed in Egypt: Health risk assessment, benzo[a]pyrene induced mutagenicity and oxidative stress in human colon (CaCo-2) cells and protection using rosmarinic and ascorbic acids

被引：36

作者：

Darwish, Wageh Sobhy ^{[1
,2
]}

Chiba, Hitoshi ^{[3
]}

El-Ghareeb, Waleed Rizk ^{[2
,4
]}

Elhelaly, Abdelazim Elsayed ^{[5
,6
]}

Hui, Shu-Ping ^{[1
]}

机构：

[1] Hokkaido Univ, Fac Hlth Sci, Lab Adv Lipid Anal, Kita 12,Nishi 5, Sapporo, Hokkaido 0600812, Japan

[2] Zagazig Univ, Fac Vet Med, Food Control Dept, Zagazig 44519, Egypt

[3] Sapporo Univ Hlth Sci, Dept Nutr, Higashi Ku, Nakanuma Nishi 4-2-1-15, Sapporo, Hokkaido 0070894, Japan

[4] King Faisal Univ, Coll Vet Med, Dept Vet Publ Hlth & Anim Husb, Al Hufuf, Saudi Arabia

[5] Suez Canal Univ, Fac Vet Med, Dept Food Hyg & Control, Ismailia 41522, Egypt

[6] Gifu Univ, Ctr Emerging Infect Dis, Sch Med, Gifu 5011193, Japan

来源：

FOOD CHEMISTRY | 2019年 / 290卷

关键词：

Polycyclic aromatic hydrocarbons; Benzo[a]pyrene; Heat-treated meat; Egypt; Rosmarinic acid; Ascorbic acid; XENOBIOTIC-METABOLIZING ENZYMES; DIETARY EXPOSURE; BETA-CAROTENE; PAHS; FOOD; CARCINOGENESIS; BENZO(A)PYRENE; CANCER; IDENTIFICATION; ACTIVATION;

D O I：

10.1016/j.foodchem.2019.03.127

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

This study was undertaken to estimate the concentrations of the formed polycyclic aromatic hydrocarbons (PAHs) in heat-treated (boiled, pan-fried and grilled) meats collected from Egypt. Dietary intakes and cancer risks of PAHs among Egyptian adults were calculated. Benzo[a]pyrene (B[a]P)-induced mutagenicity and oxidative stress in human colon (CaCo-2) cell line and mechanisms behind such effects were also investigated. Finally, protection trials using rosmarinic (RMA) and ascorbic acids (ASA) were carried out. The results indicated formation of PAHs at high levels in the heat-treated meats. Calculated incremental life time cancer risk among Egyptian adults were 7.05179E - 07, 7.00604 E - 06 and 1.86069 E - 05 due to ingestion of boiled, panfried and grilled meats, respectively. B[a]P-exposed CaCo-2 cells had high abilities for mutagenicity (490.05 +/- 21.37 His + revertants) and production of reactive oxygen species. RMA and ASA protected CaCo-2 cells via reduction of B[a]P-induced mutagenicity and oxidative stress and upregulation of phase II detoxification enzymes and xenobiotic transporters.

引用

页码：114 / 124

页数：11

共 46 条

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