Genome-Wide Identification and Characterization of the PERK Gene Family in Gossypium hirsutum Reveals Gene Duplication and Functional Divergence

被引：51

作者：

Qanmber, Ghulam ^{[1
]}

Liu, Ji ^{[1
]}

Yu, Daoqian ^{[1
]}

Liu, Zhao ^{[1
]}

Lu, Lili ^{[1
]}

Mo, Huijuan ^{[1
]}

Ma, Shuya ^{[1
]}

Wang, Zhi ^{[1
,2
]}

Yang, Zuoren ^{[1
,2
]}

机构：

[1] Chinese Acad Agr Sci, Inst Cotton Res, State Key Lab Cotton Biol, Key Lab Biol & Genet Breeding Cotton, Anyang 455000, Peoples R China

[2] Zhengzhou Univ, State Key Lab Cotton Biol, Zhengzhou Reseach Base, Zhengzhou 450000, Henan, Peoples R China

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2019年 / 20卷 / 07期

基金：

中国国家自然科学基金;

关键词：

G; hirsutum; GhPERK; sequence logos; phylogenetic analysis; cis-elements; gene duplication; abiotic stress; phytohormone stress; RECEPTOR-LIKE KINASES; COEXPRESSION NETWORKS; PROLINE-RICH; TRANSCRIPTION FACTORS; EXPRESSION ANALYSIS; ABSCISIC-ACID; ARABIDOPSIS; PROTEIN; EVOLUTION; SEQUENCE;

D O I：

10.3390/ijms20071750

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Proline-rich extensin-like receptor kinases (PERKs) are an important class of receptor kinases in plants. Receptor kinases comprise large gene families in many plant species, including the 15 PERK genes in Arabidopsis. At present, there is no comprehensive published study of PERK genes in G. hirsutum. Our study identified 33 PERK genes in G. hirsutum. Phylogenetic analysis of conserved PERK protein sequences from 15 plant species grouped them into four well defined clades. The GhPERK gene family is an evolutionarily advanced gene family that lost its introns over time. Several cis-elements were identified in the promoter regions of the GhPERK genes that are important in regulating growth, development, light responses and the response to several stresses. In addition, we found evidence for gene loss or addition through segmental or whole genome duplication in cotton. Gene duplication and synteny analysis identified 149 orthologous/paralogous gene pairs. Ka/Ks values show that most GhPERK genes experienced strong purifying selection during the rapid evolution of the gene family. GhPERK genes showed high expression levels in leaves and during ovule development. Furthermore, the expression of GhPERK genes can be regulated by abiotic stresses and phytohormone treatments. Additionally, PERK genes could be involved in several molecular, biological and physiological processes that might be the result of functional divergence.

引用

页数：23

共 89 条

[1] Approaches for extracting practical information from gene co-expression networks in plant biology [J].