Design considerations for capillary flow injection systems

Design considerations for performing capillary now injection (CFI) with conventional peristaltic pumping are presented. A time based injection technique has been developed so that sample volumes in the sub-nL to mt range can be used without internal or external injection loops. In addition, two now cells have been designed and constructed in order to measure absorbances with such small sample volumes. Commercially available connectors for fused silica capillaries have also been used in a CFI system enabling the operation of multiple reagent systems.