Chemical composition, antioxidant, anti-lipooxygenase, antimicrobial, anti-parasite and cytotoxic activities of Polyalthia longifolia seed oil

被引：7

作者：

Atolani, O. ^{[1
]}

Areh, E. T. ^{[1
]}

Oguntoye, O. S. ^{[1
]}

Zubair, M. F. ^{[2
]}

Fabiyi, O. A. ^{[3
]}

Oyegoke, R. A. ^{[4
]}

Tarigha, D. E. ^{[1
]}

Adamu, N. ^{[2
]}

Adeyemi, O. S. ^{[5
]}

Kambizi, L. ^{[6
]}

Olatunji, G. A. ^{[1
,2
]}

机构：

[1] Univ Ilorin, Dept Chem, PMB 1515, Ilorin, Nigeria

[2] Univ Ilorin, Dept Ind Chem, PMB 1515, Ilorin, Nigeria

[3] Univ Ilorin, Dept Crop Protect, PMB 1515, Ilorin, Nigeria

[4] Univ Ilorin, Dept Biochem, PMB 1515, Ilorin, Nigeria

[5] Landmark Univ, Dept Biochem, Med Biochem & Toxicol Lab, Omu Aran 251101, Kwara State, Nigeria

[6] Cape Peninsula Univ Technol, Dept Hort, Cape Town, South Africa

来源：

MEDICINAL CHEMISTRY RESEARCH | 2019年 / 28卷 / 04期

关键词：

Polyalthia longifolia; Oleic acid; Drug discovery; Medicinal chemistry; Medicinal biochemistry; Toxoplasma gondii; IN-VITRO;

D O I：

10.1007/s00044-019-02301-z

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

This work investigates the chemical compositions of Polyalthia longifolia Thw. seed oil with the associated antioxidant, anti-inflammatory, anti-parasite and cytotoxicity potentials. The oil of P. longifolia seed obtained by soxhlet extraction was trans-esterified and the fatty acid profile characterized using gas chromatography mass spectrometry (GC-MS). The antioxidant activity was evaluated using DPPH and ABTS assays. The anti-bacterial and anti-fungi properties of the oil were determine on clinical isolates of the organisms using agar diffusion method. The anti-inflammatory activities, cytotoxicity and anti-parasite potential were evaluated using lipooxygenase, mammalian cell and Toxoplasma gondii assays respectively. P. longifolia seed was observed to contain oleic (30.31%), linoleic acid (19.27%) and palmitic acid (15.11%) as the major fatty acids with low proportion of tricosylic acid (6.10) and stearic acid (5.56%). The oil had significant anti-lipooxygenase activity (IC50=0.70 +/- 0.02 mu g/mL) comparable to indomethacin (IC50=0.53 +/- 0.07 mu g/mL). The DPPH (IC50=55.91 +/- 31.18 mu g/mL) and ABTS (IC50=16.89 +/- 15.50 mu g/mL) antioxidant activity of the oil was lower to the ascorbic acid (IC50=0.34 +/- 0.04 and 0.54 +/- 0.04 mu g/mL). The oil also showed activities against all the tested bacteria and fungi. The highest inhibition was recorded against S. aureus (17 +/- 1mm) at concentration 200mg/mL. Further, the oils showed strong potential to restrict growth of Toxoplasma gondii in vitro, but the parasite growth inhibition was mildly abated in the presence of -tocopherol. The seed oil of the underutilized P. longifolia possesses essential fatty acids which could be responsible for the numerous biological potentials which include anti-lipooxygenase, antioxidant, anti-inflammatory, anti-parasite, anti-microbial and cytotoxic activities. The incorporation of the natural oil into pharmaceuticals or cosmetics may enhance antioxidant, anti-inflammatory, antimicrobial and cytotoxicity potential of such products.

引用

页码：515 / 527

页数：13

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