Polymerase chain reaction with phase change as intrinsic thermal control

被引：6

作者：

Hsieh, Yi-Fan ^{[1
]}

Yonezawa, Eri ^{[1
]}

Kuo, Long-Sheng ^{[1
]}

Yeh, Shiou-Hwei ^{[2
]}

Chen, Pei-Jer ^{[3
]}

Chen, Ping-Hei ^{[1
]}

机构：

[1] Natl Taiwan Univ, Dept Mech Engn, Taipei 106, Taiwan

[2] Natl Taiwan Univ, Grad Inst Microbiol, Taipei 106, Taiwan

[3] Natl Taiwan Univ, Grad Inst Clin Med, Taipei 106, Taiwan

来源：

APPLIED PHYSICS LETTERS | 2013年 / 102卷 / 17期

关键词：

BETA-GLOBIN; AMPLIFICATION; CONVECTION; DNA; PCR;

D O I：

10.1063/1.4803442

中图分类号：

O59 [应用物理学];

学科分类号：

摘要：

This research demonstrated that without any external temperature controller, the capillary convective polymerase chain reaction (ccPCR) powered by a candle can operate with the help of phase change. The candle ccPCR system productively amplified hepatitis B virus 122 base-pairs DNA fragment. The detection sensitivity can achieve at an initial DNA concentration to 5 copies per reaction. The results also show that the candle ccPCR system can operate functionally even the ambient temperature varies from 7 degrees C to 45 degrees C. These features imply that the candle ccPCR system can provide robust medical detection services. (C) 2013 AIP Publishing LLC.

引用

页数：3

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