Metabolic Profiling of Lignans and Other Secondary Metabolites from Rapeseed (Brassica napus L.)

被引：14
作者：
Fang, Jingjing [1 ]
Reichelt, Michael [1 ]
Kai, Marco [1 ]
Schneider, Bernd [1 ]
机构：
[1] Max Planck Inst Chem Ecol, D-07745 Jena, Germany
来源：
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2012年 / 60卷 / 42期
关键词：
Brassica napus; dienolignan derivatives; glucosinolates; lignans; rapeseed; sinapates; thomasidioic acid derivatives; ARABIDOPSIS-THALIANA; SEED; SINAPINE; ACID; ESTERS; IDENTIFICATION; BIOSYNTHESIS; CHOLINE; JUNCEA; LEAVES;
D O I：
10.1021/jf303907b
中图分类号：
S [农业科学];
学科分类号：
09 ;
摘要：
A metabolic profiling study was carried out on rapeseed (Brassica napus L.). Eleven glucosinolates were identified by high-performance liquid chromatography (HPLC) coupled with diode array detection (DAD) and mass spectrometry (MS). Additionally, 18 phenolic compounds were profiled from an ethanol extract of rapeseed. Besides two major phenols, sinapine and methyl sinapate, 16 minor phenolic compounds were isolated and identified. Seven of them are new lignans including three (+/-)-thomasidioic acid derivatives and four (E,E)-dienolignan derivatives. The structures of novel phenolic compounds were elucidated by ID and 2D nuclear magnetic resonance (NMR) spectroscopy and MS. The analytical data of secondary metabolites in rapeseed winter cultivar "Emerald" and information about purification on a microscale are useful for upcoming studies on tissue-specific localization of these compounds.
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收藏
页码：10523 / 10529
页数：7
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