Circular RNA Expression Profile of Pancreatic Ductal Adenocarcinoma Revealed by Microarray

被引：145

作者：

Li, Haimin ^{[1
]}

Hao, Xiaokun ^{[1
,2
]}

Wang, Huimin ^{[1
]}

Liu, Zhengcai ^{[1
]}

He, Yong ^{[1
]}

Pu, Meng ^{[1
]}

Zhang, Hongtao ^{[1
]}

Yu, Hengchao ^{[1
]}

Duan, Juanli ^{[1
]}

Qu, Shibin ^{[1
]}

机构：

[1] Fourth Mil Med Univ, Xijing Hosp, Dept Hepatobiliary Surg, Xian 710032, Peoples R China

[2] 141 Hosp, Dept Gen Surg, Xian, Peoples R China

来源：

CELLULAR PHYSIOLOGY AND BIOCHEMISTRY | 2016年 / 40卷 / 06期

基金：

中国国家自然科学基金;

关键词：

Circular RNA; Noncoding RNA; Pancreatic cancer; Microarray; LONG NONCODING RNAS; CANCER CELLS; TUMOR-GROWTH; INHIBITION; PROGRESSION; MICRORNA; ABUNDANT; PATHWAY; BIOLOGY;

D O I：

10.1159/000453186

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Background/Aims: Circular RNAs (circRNAs) are a special novel type of a stable, diverse and conserved noncoding RNA in mammalian cells. Particularly in cancer, circRNAs have been reported to be widely involved in the physiological/pathological process of life. However, it is unclear whether circRNAs are specifically involved in pancreatic ductal adenocarcinoma (PDAC). Methods: We investigated the expression profile of circRNAs in six PDAC cancer samples and paired adjacent normal tissues using microarray. A high-throughput circRNA microarray was used to identify dysregulated circular RNAs in six PDAC patients. Bioinformatic analyses were applied to study these differentially expressed circRNAs. Furthermore, quantitative reverse transcription polymerase chain reaction (qRT-PCR) was performed to confirm these results. Results: We revealed and confirmed that a number of circRNAs were dysregulated, which suggests a potential role in pancreatic cancer. Conclusions: this study demonstrates that clusters of circRNAs are aberrantly expressed in PDAC compared with normal samples and provides new potential targets for the future treatment of PDAC and novel insights into PDAC biology. 2016 The Author(s) Published by S. Karger AG, Basel

引用

页码：1334 / 1344

页数：11

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