UV light-emitting diode-induced fluorescence detection combined with online sample concentration techniques for capillary electrophoresis

被引：16

作者：

Chang, YS ^{[1
]}

Shih, CM ^{[1
]}

Lin, CH ^{[1
]}

机构：

[1] Natl Taiwan Normal Univ, Dept Chem, Taipei, Taiwan

来源：

ANALYTICAL SCIENCES | 2006年 / 22卷 / 02期

关键词：

D O I：

10.2116/analsci.22.235

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The application of an ultraviolet (UV) light-emitting diode (LED) to on-line sample concentration/fluorescence detection in capillary electrophoresis (CE) is described. The utility of a UV-LED (peak emission wavelength at 380 nm, similar to 2 mW) for fluorescence detection was demonstrated by examining both a naturally fluorescent (riboflavin) compound and a nonfluorescent compound (tryptophan), respectively. The detection limit for riboflavin was determined to be 0.2 ppm by the normal MEKC mode, which was improved to 3 - 7 ppb when dynamic pH-junction technique was applied. On the other hand, the detection limit of the tryptophan derivative was determined to be 1.5 ppm using the MEKC mode, which was improved to 3 ppb when the sweeping-MEKC mode was applied. In an analysis of an actual sample, the concentrations of riboflavin in beer, and tryptophan in urine and milk samples were determined, respectively.

引用

页码：235 / 240

页数：6

共 33 条

[1] Response to comment on "Electrokinetic stacking injection of neutral analytes under continuous conductivity conditions" [J].