A High Performance Liquid Chromatographic Method Using UV Detection for the Determination of Lisinopril

A fast and simple HPLC method for the determination of lisinopril dissolved in pH 1.2 buffer was developed and validated. UV detection at 215 nm was used, on a HPLC Agilent 1200 system. Lisinopril was separated on a CS column using a mobile phase consisting of a mixture of 0.125% sodium hexanesulphonate solution prepared in phosphate buffer pH 2 and acetonitrile (72:28, v/v), at a flow rate of I mL.min(-1). The injected volume was 20 mu L. In these chromatographic conditions, the retention time of lisinopril was 1.9 minutes and the overall time of the analysis was 3 minutes. The proposed method was validated for a linear range of 0.6-60 mu g.mL(-1) with correlation coefficient of r = 0.9991. The precision and accuracy were within 1.3 % for inter- and intra-day HPLC runs. The overall recovery for lisinopril was 99%. This assay can be used for the therapeutic drug monitoring of lisinopril.