Responses of flocculated activated sludge to bimetallic Ag-Fe nanoparticles toxicity: Performance, activity enzymatic, and bacterial community shift

Ever-increasing production and use of nanoparticles (NPs) have aroused overarching concerns for their toxic effects on the environment and human. In the present study, the toxic effects of Silver (Ag) and Iron (Fe) NPs on the performance of activated sludge were investigated under continuous aerobic/anoxic/anaerobic conditions in laboratory-scale sequencing batch reactors (SBRs).Activated sludge was exposed to various concentrations (5-100 mg/L) of Ag-Fe NPs for 60 days and its response was assessed through the enzymatic activity, COD, nitrogen (TN) and phosphorus (TP) removal, toxicity tests, as well as variations in bacterial community. Compared with the pristine control sample, the exposure to NPs suppressed TN and TP removal efficiencies. Indeed, the respiration rate and biomass concentration were significantly affected by the NPs. Although the simultaneous exposure to Ag-Fe NPs did affect the integrity of cell membrane (LDH) and key enzymes activities, the higher concentration induced an increased generation of reactive oxygen species (ROS). The metagenome analysis revealed a marked shift in the microbial community structure suggesting that both heterotrophic and autotrophic communities were affected by the presence of Ag-Fe NPs. Our results provide some evidence for compounded effects of NPs in their simultaneous presence, and generate new leads for future research efforts.