Identification of very-long-chain polyunsaturated fatty acids from Amphidinium carterae by atmospheric pressure chemical ionization liquid chromatography-mass spectroscopy

被引：22
作者：
Rezanka, Tomas [1 ]
Nedbalova, Linda [2 ,3 ]
Sigler, Karel [1 ]
机构：
[1] Acad Sci Czech Republ, Inst Microbiol, CR-14220 Prague, Czech Republic
[2] Charles Univ Prague, Dept Ecol, Fac Sci, CR-12844 Prague 2, Czech Republic
[3] Acad Sci Czech Republ, Inst Bot, CS-37982 Trebon, Czech Republic
来源：
PHYTOCHEMISTRY | 2008年 / 69卷 / 12期
关键词：
Amphidinium carterae; very-long-chain polyunsaturated fatty acids; liquid chromatography-mass spectrometry - atmospheric pressure chemical ionization;
D O I：
10.1016/j.phytochem.2008.06.011
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A method is described for the enrichment of very-long-chain polyunsaturated fatty acids (VLCPUFAs) from total fatty acids of Amphidinium carterae and their identification as picolinyl esters by means of microbore liquid chromatography-mass spectrometry with atmospheric pressure chemical ionization (LC-MS/APCI). The combination of argentation TLC and LC-MS/APCI was used to identify unusual VLCPUFAs up to hexatriacontaoctaenoic acid. Two acids, 36:7n-6 and 36:8n-3, were also synthesized to unambiguously confirm their structure. The possibilities of VLCPUFAs biosynthesis are proposed. (C) 2008 Elsevier Ltd. All rights reserved.
引用
收藏
页码：2391 / 2399
页数：9
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