Early stage of cytomegalovirus infection suppresses host microRNA expression regulation in human fibroblasts

被引：11

作者：

Buzdin, Anton A. ^{[1
,2
,3
]}

Artcibasova, Alina V. ^{[1
,4
]}

Fedorova, Natalya F. ^{[5
]}

Suntsova, Maria V. ^{[1
,2
]}

Garazha, Andrew V. ^{[1
,6
]}

Sorokin, Maxim I. ^{[7
]}

Allina, Daria ^{[6
,7
]}

Shalatonin, Mikhail ^{[8
]}

Borisov, Nikolay M. ^{[3
,7
]}

Zhavoronkov, Alex A. ^{[1
,4
]}

Kovalchuk, Igor ^{[9
]}

Kovalchuk, Olga ^{[9
]}

Kushch, Alla A. ^{[5
]}

机构：

[1] D Rogachyov Fed Res Ctr Pediat Hematol Oncol & Im, Lab Bioinformat, Moscow, Russia

[2] Shemyakin Ovchinnikov Inst Bioorgan Chem, Grp Genom Regulat Cell Signaling Syst, Moscow, Russia

[3] Natl Res Ctr Kurchatov Inst, Ctr Convergence Nano Bio Informat & Cognit Sci &, Moscow, Russia

[4] Pathway Pharmaceut, Hong Kong, Hong Kong, Peoples R China

[5] Minist Hlth Russian Federat, NF Gamaleya Fed Res Ctr Epidemiol & Microbiol, Moscow, Russia

[6] Moscow Inst Phys & Technol, Dolgoprudnyi, Moscow Region, Russia

[7] First Oncol Res & Advisory Ctr, Moscow, Russia

[8] Morozov Childrens City Hosp, Moscow, Russia

[9] Univ Lethbridge, Dept Biol Sci, Lethbridge, AB, Canada

来源：

CELL CYCLE | 2016年 / 15卷 / 24期

关键词：

Cytomegalovirus infection; gene transcription; human fibroblasts; intracellular signaling pathway regulation; microRNA; total miRNAome; SIGNALING PATHWAY ACTIVATION; HUMAN BLADDER-CANCER; CELLS; RNAS; TRANSCRIPTION; DISEASE; MODELS; AGE;

D O I：

10.1080/15384101.2016.1241928

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Responses to human cytomegalovirus (HCMV) infection are largely individual and cell type specific. We investigated molecular profiles in 2 primary cell cultures of human fibroblasts, which are highly or marginally sensitive to HCMV infection, respectively. We screened expression of genes and microRNAs (miRs) at the early (3 hours) stage of infection. To assess molecular pathway activation profiles, we applied bioinformatic algorithms OncoFinder and MiRImpact. In both cell types, pathway regulation properties at mRNA and miR levels were markedly different. Surprisingly, in the infected highly sensitive cells, we observed a "freeze" of miR expression profiles compared to uninfected controls. Our results evidence that in the sensitive cells, HCMV blocks intracellular regulation of microRNA expression already at the earliest stage of infection. These data suggest somewhat new functions for HCMV products and demonstrate dependence of miR expression arrest on the host-encoded factors.

引用

页码：3378 / 3389

页数：12

共 42 条

[1] Large-scale profiling of signalling pathways reveals an asthma specific signature in bronchial smooth muscle cells [J].

Alexandrova, Elena ;

Nassa, Giovanni ;

Corleone, Giacomo ;

Buzdin, Anton ;

Aliper, Alexander M. ;

Terekhanova, Nadezhda ;

Shepelin, Denis ;

Zhavoronkov, Alexander ;

Tamm, Michael ;

Milanesi, Luciano ;

Miglino, Nicola ;

Weisz, Alessandro ;

Borger, Pieter .

ONCOTARGET, 2016, 7 (18) :25150-25161

[2] Signaling pathway activation drift during aging: Hutchinson-Gilford Progeria Syndrome fibroblasts are comparable to normal middle-age and old-age cells [J].

Aliper, Alexander M. ;

Csoka, Antonei Benjamin ;

Buzdin, Anton ;

Jetka, Tomasz ;

Roumiantsev, Sergey ;

Moskalev, Alexey ;

Zhavoronkov, Alex .

AGING-US, 2015, 7 (01) :26-37

[3] Interactome analysis of myeloid-derived suppressor cells in murine models of colon and breast cancer [J].

Aliper, Alexander M. ;

Frieden-Korovkina, Victoria P. ;

Buzdin, Anton ;

Roumiantsev, Sergey A. ;

Zhavoronkov, Alex .

ONCOTARGET, 2014, 5 (22) :11345-11353

[4] MiRImpact, a new bioinformatic method using complete microRNA expression profiles to assess their overall influence on the activity of intracellular molecular pathways [J].

Artcibasova, Alina V. ;

Korzinkin, Mikhail B. ;

Sorokin, Maksim I. ;

Shegay, Peter V. ;

Zhavoronkov, Alex A. ;

Gaifullin, Nurshat ;

Alekseev, Boris Y. ;

Vorobyev, Nikolay V. ;

Kuzmin, Denis V. ;

Kaprind, Andrey D. ;

Borisov, Nikolay M. ;

Buzdin, Anton A. .

CELL CYCLE, 2016, 15 (05) :689-698

[5] The life cycle and pathogenesis of human cytomegalovirus infection: lessons from proteomics [J].

Beltran, Pierre M. Jean ;

Cristea, Ileana M. .

EXPERT REVIEW OF PROTEOMICS, 2014, 11 (06) :697-711

[6]

Brennecke J, 2007, CELL, V128, P1089, DOI 10.1016/j.cell.2007.01.043

[7] Vaccines against human cytomegalovirus: Time to test [J].

Britt, WJ .

TRENDS IN MICROBIOLOGY, 1996, 4 (01) :34-38

[8] Altered cellular mRNA levels in human cytomegalovirus-infected fibroblasts: Viral block to the accumulation of antiviral mRNAs [J].

Browne, EP ;

Wing, B ;

Coleman, D ;

Shenk, T .

JOURNAL OF VIROLOGY, 2001, 75 (24) :12319-12330

[9]

Buzdin Anton A, 2014, Front Mol Biosci, V1, P8, DOI 10.3389/fmolb.2014.00008

[10] Oncofinder, a new method for the analysis of intracellular signaling pathway activation using transcriptomic data [J].

Buzdin, Anton A. ;

Zhavoronkov, Alex A. ;

Korzinkin, Mikhail B. ;

Venkova, Larisa S. ;

Zenin, Alexander A. ;

Smirnov, Philip Yu. ;

Borisov, Nikolay M. .

FRONTIERS IN GENETICS, 2014, 5

← 1 2 3 4 5 →