Ras protein of the slime mold Physarum polycephalum is farnesylated in vitro

被引：1
作者：
Talarczyk, E
Hung, VS
Kozlowski, P
Trzcinska-Danielewicz, J
Swiezewska, E
机构：
[1] Polish Acad Sci, Inst Biochem & Biophys, PL-02106 Warsaw, Poland
[2] Univ Warsaw, Inst Biochem, PL-02089 Warsaw, Poland
来源：
ACTA BIOCHIMICA POLONICA | 1999年 / 46卷 / 03期
关键词：
protein farnesyltransferase; protein geranylgeranyl transferase I; prenylation; spinach; Physarum polycephalum;
D O I：
10.18388/abp.1999_4149
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Physarum Ppras1 protein was efficiently prenylated by prenyltransferases of spinach. Surprisingly in spite of the C-terminal sequence (CLLL) specific for geranylgeranylation the protein was preferentially farnesylated. Consequences of this observation are discussed.
引用
收藏
页码：771 / 775
页数：5
相关论文
共 18 条
[1]  
BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[2]   A SIMPLE EFFICIENT LIQUID SCINTILLATOR FOR COUNTING AQUEOUS SOLUTIONS IN A LIQUID SCINTILLATION COUNTER [J].
BRAY, GA .
ANALYTICAL BIOCHEMISTRY, 1960, 1 (4-5) :279-285
[3]   FUNGAL LIPOPEPTIDE MATING PHEROMONES - A MODEL SYSTEM FOR THE STUDY OF PROTEIN PRENYLATION [J].
CALDWELL, GA ;
NAIDER, F ;
BECKER, JM .
MICROBIOLOGICAL REVIEWS, 1995, 59 (03) :406-422
[4]   Protein prenyltransferases [J].
Casey, PJ ;
Seabra, MC .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (10) :5289-5292
[5]   PROTEIN ISOPRENYLATION AND METHYLATION AT CARBOXYL-TERMINAL CYSTEINE RESIDUES [J].
CLARKE, S .
ANNUAL REVIEW OF BIOCHEMISTRY, 1992, 61 :355-386
[6]   A protein farnesyl transferase involved in abscisic acid signal transduction in Arabidopsis [J].
Cutler, S ;
Ghassemian, M ;
Bonetta, D ;
Cooney, S ;
McCourt, P .
SCIENCE, 1996, 273 (5279) :1239-1241
[7]   POLYLYSINE AND CVIM SEQUENCES OF K-RASB DICTATE SPECIFICITY OF PRENYLATION AND CONFER RESISTANCE TO BENZODIAZEPINE PEPTIDOMIMETIC IN-VITRO [J].
JAMES, GL ;
GOLDSTEIN, JL ;
BROWN, MS .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (11) :6221-6226
[8]   REGIONS OUTSIDE OF THE CAAX MOTIF INFLUENCE THE SPECIFICITY OF PRENYLATION OF G-PROTEIN GAMMA-SUBUNITS [J].
KALMAN, VK ;
ERDMAN, RA ;
MALTESE, WA ;
ROBISHAW, JD .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (24) :14835-14841
[9]   IDENTIFICATION OF A RAS GENE IN THE SLIME-MOLD PHYSARUM-POLYCEPHALUM [J].
KOZLOWSKI, P ;
FRONK, J ;
TOCZKO, K .
BIOCHIMICA ET BIOPHYSICA ACTA, 1993, 1173 (03) :357-359
[10]   Identification of spinach farnesyl protein transferase - Dithiothreitol as an acceptor in vitro [J].
Parmryd, I ;
Shipton, CA ;
Swiezewska, E ;
Andersson, B ;
Dallner, G .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1995, 234 (03) :723-731
12