A vaccinia-based single vector construct multi-pathogen vaccine protects against both Zika and chikungunya viruses

被引：67

作者：

Prow, Natalie A. ^{[1
,2
,3
]}

Liu, Liang ^{[4
]}

Nakayama, Eri ^{[1
,5
]}

Cooper, Tamara H. ^{[4
]}

Yan, Kexin ^{[1
]}

Eldi, Preethi ^{[4
]}

Hazlewood, Jessamine E. ^{[1
]}

Tang, Bing ^{[1
]}

Le, Thuy T. ^{[1
]}

Setoh, Yin Xiang ^{[6
]}

Khromykh, Alexander A. ^{[2
,3
,6
]}

Hobson-Peters, Jody ^{[6
]}

Diener, Kerrilyn R. ^{[4
,7
,8
]}

Howley, Paul M. ^{[9
]}

Hayball, John D. ^{[4
,7
,8
]}

Suhrbier, Andreas ^{[1
,2
,3
]}

机构：

[1] QIMR Berghofer Med Res Inst, Brisbane, Qld 4029, Australia

[2] Australian Infect Dis Res Ctr, Brisbane, Qld 4029, Australia

[3] Australian Infect Dis Res Ctr, Brisbane, Qld 4072, Australia

[4] Univ South Australia, Inst Hlth Res, Sch Pharm & Med Sci, Expt Therapeut Lab, Adelaide, SA 5000, Australia

[5] Natl Inst Infect Dis, Dept Virol 1, Tokyo 1628640, Japan

[6] Univ Queensland, Sch Chem & Mol Biosci, St Lucia, Qld 4072, Australia

[7] Univ Adelaide, Robinson Res Inst, Adelaide, SA 5005, Australia

[8] Univ Adelaide, Adelaide Med Sch, Adelaide, SA 5005, Australia

[9] Sementis Ltd, Berwick, Vic 3806, Australia

来源：

NATURE COMMUNICATIONS | 2018年 / 9卷

基金：

澳大利亚研究理事会; 英国医学研究理事会;

关键词：

HUMORAL IMMUNITY; IN-VIVO; INFECTION; CELL; VACCINATIONS; TRANSMISSION; RESPONSES; CAPACITY; STRAIN; DENGUE;

D O I：

10.1038/s41467-018-03662-6

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Zika and chikungunya viruses have caused major epidemics and are transmitted by Aedes aegypti and/or Aedes albopictus mosquitoes. The "Sementis Copenhagen Vector" (SCV) system is a recently developed vaccinia-based, multiplication-defective, vaccine vector technology that allows manufacture in modified CHO cells. Herein we describe a single-vector construct SCV vaccine that encodes the structural polyprotein cassettes of both Zika and chikungunya viruses from different loci. A single vaccination of mice induces neutralizing antibodies to both viruses in wild-type and IFNAR(-/-) mice and protects against (i) chikungunya virus viremia and arthritis in wild-type mice, (ii) Zika virus viremia and fetal/placental infection in female IFNAR(-/-) mice, and (iii) Zika virus viremia and testes infection and pathology in male IFNAR(-/-) mice. To our knowledge this represents the first single-vector construct, multi-pathogen vaccine encoding large polyproteins, and offers both simplified manufacturing and formulation, and reduced "shot burden" for these often co-circulating arboviruses.

引用

页数：12

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