Pharmacokinetics of Panasenoside in Rats and Tissue Distribution in Mice by Ultra-Performance Liquid Chromatography Tandem Mass Spectrometry

We developed an ultra-performance liquid chromatography tandem mass spectrometry (UPLC-MS/MS) method for quantification of panasenoside pharmacokinetics in rat plasma and tissue distribution in mouse. Twelve male Sprague-Dawley rats were used for pharmacokinetics after intravenous (2 or 10 mg/kg) administration of panasenoside, six rats for each dose. Thirty mice were randomly divided into six groups (five mice for each group, one group for each time point) and received 20 mg/kg of panasenoside by intraperitoneal administration. Calibration plots were in the range of 2-2000 ng/mL for panasenoside in rat plasma and 2-3000 ng/mL in mouse tissues. The relative standard deviation (RSD) of inter-day and intra-day precision was less than 14%. The accuracy was between 89.6% and 110.0%. The AUC((0-t)) was 160.8 +/- 13.0 and 404.9 +/- 78.0 ng/mL*h, and t(1/2) of 3.2 +/- 1.2 and 4.6 +/- 1.7 h, CL (clearance) of 10.0 +/- 2.0, and 21.4 +/- 2.0 L/h/kg after intravenous administration 2 mg/kg and 10 mg/kg of panasenoside, respectively. The tissue distribution results indicated that the panasenoside diffuses rapidly and widely into major organs. The level of panasenoside was highest in mouse liver, followed by kidney, lung, and spleen. The overwhelming accumulation in liver indicated that liver was responsible for the extensive metabolism.