Paracellular Barrier and Tight Junction Protein Expression in the Immortalized Brain Endothelial Cell Lines bEND.3, bEND.5 and Mouse Brain Endothelial Cell 4

被引：96
作者：
Watanabe, Takuya [1 ,2 ]
Dohgu, Shinya [1 ]
Takata, Fuyuko [1 ]
Nishioku, Tsuyoshi [1 ]
Nakashima, Akio [1 ,2 ]
Futagami, Koujiro [2 ]
Yamauchi, Atsushi [1 ]
Kataoka, Yasufumi [1 ]
机构：
[1] Fukuoka Univ, Fac Pharmaceut Sci, Dept Pharmaceut Care & Hlth Sci, Jonan Ku, Fukuoka 8140180, Japan
[2] Fukuoka Univ, Fac Pharmaceut Sci, Dept Pharmaceut & Hlth Care Management, Jonan Ku, Fukuoka 8140180, Japan
来源：
BIOLOGICAL & PHARMACEUTICAL BULLETIN | 2013年 / 36卷 / 03期
基金：
日本学术振兴会;
关键词：
blood-brain barrier; tight junction; brain endothelial cell; PERMEABILITY; MODEL; RAT;
D O I：
10.1248/bpb.b12-00915
中图分类号：
R9 [药学];
学科分类号：
1007 ;
摘要：
The blood brain barrier (BBB) is formed by brain endothelial cells. Many immortalized brain endothelial cell lines have been established; these have been used as in vitro BBB models. The aim of the present study was to assess the paracellular barrier properties of the immortalized mouse brain endothelial cell lines bEND.3, bEND.5 cells, and mouse brain endothelial cell 4 (MBEC4), and those of the primary mouse brain endothelial cells pMBECs. bEND.3 cells showed low permeability to sodium fluorescein and obvious staining of tight junction proteins (claudin-5, occludin and ZO-1) similar to pMBECs; these barrier properties of MBEC4 and bEND.5 cells were low. In addition, bEND.3 cells expressed the highest level of claudin-5 among all cells. These results suggest that bEND.3 cells are a convenient and useful model for evaluating BBB function, especially the paracellular barrier.
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收藏
页码：492 / 495
页数：4
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