First Live Birth in Germany after Re-Transplantation of Cryopreserved Ovarian Tissue: Original Device for Initiation of Ice Formation

被引：23

作者：

Isachenko, Vladimir ^{[1
]}

Isachenko, Evgena ^{[1
]}

Keck, Gudrun ^{[2
]}

Dittrich, Ralf ^{[3
]}

Montag, Markus ^{[4
]}

van der Ven, Hans ^{[5
]}

Mallmann, Peter ^{[1
]}

Mueller, Andreas ^{[3
]}

Distler, Wolfgang ^{[2
]}

Beckmann, Matthias W. ^{[3
]}

Rahimi, Gohar ^{[1
]}

机构：

[1] Univ Frauenklin Cologne, Cologne, Germany

[2] Univ Frauenklin Dresden, Dresden, Germany

[3] Univ Frauenklin Erlangen, Erlangen, Germany

[4] Univ Frauenklin Heidelberg, Heidelberg, Germany

[5] Univ Frauenklin Bonn, Bonn, Germany

来源：

CLINICAL LABORATORY | 2012年 / 58卷 / 9-10期

关键词：

human; ovarian tissue; cooling; cryopreservation; ice-formation; live birth; FERTILITY PRESERVATION; AUTOTRANSPLANTATION; CANCER; VITRIFICATION; MORPHOLOGY; PREGNANCY; FOLLICLES; FAILURE; PATIENT; TUMORS;

D O I：

10.7754/Clin.Lab.2012.120224

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Background: The problem of post-cancer infertility is of significant concern. The cryopreservation of ovarian tissue before cancer therapy with retransplantation after convalescence is the key to solving this problem. Methods: Cryopreservation of ovarian tissue was performed in 2005 after surgical operation, post-operative low-temperature 22 hour transportation, and freezing using a special, original design block constructed for the initiation of ice formation (ice-seeding). We present the construction and function of this block. Results: In 2011, it was noted that a baby was born after thawing and re-transplantation of ovarian tissue. The technical and biological aspects of initiated crystals formation in the process of cryopreservation are emphasised and discussed. Conclusions: The first live birth in Germany after re-transplantation of cryopreserved ovarian tissue was noted. This cryopreservation was performed using the protocol described here. Block for auto-seeding of principally new construction recommended. (Clin. Lab. 2012;58:933-938. DOI: 10.7754/Clin.Lab.2012.120224)

引用

页码：933 / 938

页数：6

共 33 条

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