First Live Birth in Germany after Re-Transplantation of Cryopreserved Ovarian Tissue: Original Device for Initiation of Ice Formation

被引:23
|
作者
Isachenko, Vladimir [1 ]
Isachenko, Evgena [1 ]
Keck, Gudrun [2 ]
Dittrich, Ralf [3 ]
Montag, Markus [4 ]
van der Ven, Hans [5 ]
Mallmann, Peter [1 ]
Mueller, Andreas [3 ]
Distler, Wolfgang [2 ]
Beckmann, Matthias W. [3 ]
Rahimi, Gohar [1 ]
机构
[1] Univ Frauenklin Cologne, Cologne, Germany
[2] Univ Frauenklin Dresden, Dresden, Germany
[3] Univ Frauenklin Erlangen, Erlangen, Germany
[4] Univ Frauenklin Heidelberg, Heidelberg, Germany
[5] Univ Frauenklin Bonn, Bonn, Germany
关键词
human; ovarian tissue; cooling; cryopreservation; ice-formation; live birth; FERTILITY PRESERVATION; AUTOTRANSPLANTATION; CANCER; VITRIFICATION; MORPHOLOGY; PREGNANCY; FOLLICLES; FAILURE; PATIENT; TUMORS;
D O I
10.7754/Clin.Lab.2012.120224
中图分类号
R446 [实验室诊断]; R-33 [实验医学、医学实验];
学科分类号
1001 ;
摘要
Background: The problem of post-cancer infertility is of significant concern. The cryopreservation of ovarian tissue before cancer therapy with retransplantation after convalescence is the key to solving this problem. Methods: Cryopreservation of ovarian tissue was performed in 2005 after surgical operation, post-operative low-temperature 22 hour transportation, and freezing using a special, original design block constructed for the initiation of ice formation (ice-seeding). We present the construction and function of this block. Results: In 2011, it was noted that a baby was born after thawing and re-transplantation of ovarian tissue. The technical and biological aspects of initiated crystals formation in the process of cryopreservation are emphasised and discussed. Conclusions: The first live birth in Germany after re-transplantation of cryopreserved ovarian tissue was noted. This cryopreservation was performed using the protocol described here. Block for auto-seeding of principally new construction recommended. (Clin. Lab. 2012;58:933-938. DOI: 10.7754/Clin.Lab.2012.120224)
引用
收藏
页码:933 / 938
页数:6
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