Cell viability and adhesion on diamond-like carbon films containing titanium dioxide nanoparticles

被引：29

作者：

Wachesk, C. C. ^{[1
]}

Pires, C. A. F. ^{[1
]}

Ramos, B. C. ^{[2
]}

Trava-Airoldi, V. J. ^{[3
]}

Lobo, A. O. ^{[2
]}

Pacheco-Soares, C. ^{[1
]}

Marciano, F. R. ^{[2
]}

Da-Silva, N. S. ^{[1
]}

机构：

[1] Univ Vale Paraiba Univap, Lab Biomed Nanotechnol NanoBio, Lab Biol Celular & Tecidual, BR-12244000 Sao Jose Dos Campos, SP, Brazil

[2] Univ Vale Paraiba Univap, Lab Biomed Nanotechnol NanoBio, BR-12244000 Sao Jose Dos Campos, SP, Brazil

[3] INPE, Associated Lab Sensors & Mat, BR-12227010 Sao Jose Dos Campos, SP, Brazil

来源：

APPLIED SURFACE SCIENCE | 2013年 / 266卷

基金：

巴西圣保罗研究基金会;

关键词：

Diamond-like carbon; TiO2; nanoparticles; Cell viability; Fibroblasts; SURFACE FREE-ENERGY; THERMODYNAMIC ASPECTS; ION-IMPLANTATION; POLYMER SURFACES; CYTO-TOXICITY; DLC COATINGS; DEPOSITION; BIOCOMPATIBILITY; WETTABILITY; GROWTH;

D O I：

10.1016/j.apsusc.2012.11.124

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

The combination of low friction, wear resistance, high hardness, biocompatibility and chemical inertness makes diamond-like carbon (DLC) films suitable in a numerous applications in biomedical engineering. The cell viability and adhesion of L929 mouse fibroblasts was investigated using two different colorimetric assays: (i) 2-(4,5-dimethyl-2-thiazolyl)-3,5-diphenyl-2H-tetrazolium bromide (MTT), and (ii) lactate dehydrogenase (LDH). The films were growth on 316L stainless steel substrates using plasma enhanced chemical vapor deposition technique from a dispersion of TiO2 nanopowder in hexane. The increasing concentration of TiO2 nanoparticles in DLC films enhanced the mitochondrial activity and decreases the LDH activity on these samples. Fluorescence and scanning electron microscopy corroborate the results. These experiments show the potential use of DLC and TiO2-DLC films in biomedical applications. (c) 2012 Elsevier B.V. All rights reserved.

引用

页码：176 / 181

页数：6

共 48 条

[1] Amorphous carbonated apatite formation on diamond-like carbon containing titanium oxide [J].