Adoptive transfer of transplantation tolerance mediated by CD4+CD25+ and CD8+CD28- regulatory T cells induced by anti-donor-specific T-cell vaccination
被引:8
作者:
Wang, J.
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机构:
Huadong Res Inst Med & Biotech, Nanjing, Peoples R ChinaS China Univ Technol, Sch Biosci & Bioengn, Prov Key Lab Biotechnol, Guangzhou 510640, Peoples R China
Wang, J.
[2
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Zhang, L.
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Mt Sinai Sch Med, New York, NY USAS China Univ Technol, Sch Biosci & Bioengn, Prov Key Lab Biotechnol, Guangzhou 510640, Peoples R China
Zhang, L.
[3
]
Tang, J.
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机构:S China Univ Technol, Sch Biosci & Bioengn, Prov Key Lab Biotechnol, Guangzhou 510640, Peoples R China
Tang, J.
Jiang, S.
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机构:
New York Blood Ctr, Lindsley F Kimball Res Inst, New York, NY 10021 USAS China Univ Technol, Sch Biosci & Bioengn, Prov Key Lab Biotechnol, Guangzhou 510640, Peoples R China
Jiang, S.
[4
]
Wang, X.
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S China Univ Technol, Sch Biosci & Bioengn, Prov Key Lab Biotechnol, Guangzhou 510640, Peoples R ChinaS China Univ Technol, Sch Biosci & Bioengn, Prov Key Lab Biotechnol, Guangzhou 510640, Peoples R China
Wang, X.
[1
]
机构:
[1] S China Univ Technol, Sch Biosci & Bioengn, Prov Key Lab Biotechnol, Guangzhou 510640, Peoples R China
[2] Huadong Res Inst Med & Biotech, Nanjing, Peoples R China
[3] Mt Sinai Sch Med, New York, NY USA
[4] New York Blood Ctr, Lindsley F Kimball Res Inst, New York, NY 10021 USA
Previous studies have shown that vaccinating rodents with anti-donor-specific T cells significantly prolonged allograft survival; however, the putative mechanism of the tolerance remains unclear. In this study, we used the model of heterotopic heart transplantation between the C57BL/6 donor mice and BALB/c recipient mice vaccinated with anti-donor (C57BL/6) or anti-third party (C3H)-specific T cells to determine whether T cells prolong survival of mouse heart allografts and which cells were involved in induction of allograft tolerance. We observed that the mean survival time (MST) of C57BL/6 heart grafts in BALB/c mice vaccinated with anti-C57BL/6 specific T cells (43.1 +/- 4.7 days) was prolonged from that in untreated BALB/c mice (9.5 +/- 1.1 days) or BALB/c mice receiving anti-C3H-specific T cells (10.4 +/- 1.9 days). These results suggested that alloantigen-specific T-cell vaccination significantly prolonged cardiac allograft survival. The CD4(+)CD25(+) or CD8(+)CD28(-) T cells purified from splenocytes of BALB/c mice vaccinated with anti-donor-specific T cells proliferated markedly in response to irradiated anti-C57BL/6 -specific T cells in vitro. Adoptive transfer of these CD4(+)CD25(+) or CD8(+)CD28(-) T cells to naive syngenic mice significantly prolonged the survival of heart allografts. These data suggested that anti-donor-specific T-cell vaccination induced development of CD4(+)CD25(+) or CD8(+)CD28(-) regulatory T cells, which in turn mediated allogeneic-specific tolerance.
机构:
Imperial Coll, Hammersmith Hosp, Dept Hematol, Fac Med, Du Cane Rd, London W12 0NN, EnglandImperial Coll, Hammersmith Hosp, Dept Hematol, Fac Med, Du Cane Rd, London W12 0NN, England
Nadal, Elisabet
Longinotti, Maurizio
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Univ Sassari, Inst Hematol, I-07100 Sassari, ItalyImperial Coll, Hammersmith Hosp, Dept Hematol, Fac Med, Du Cane Rd, London W12 0NN, England
Longinotti, Maurizio
Dazzi, Francesco
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Imperial Coll, Hammersmith Hosp, Dept Hematol, Fac Med, Du Cane Rd, London W12 0NN, EnglandImperial Coll, Hammersmith Hosp, Dept Hematol, Fac Med, Du Cane Rd, London W12 0NN, England
机构:
Imperial Coll, Hammersmith Hosp, Dept Hematol, Fac Med, Du Cane Rd, London W12 0NN, EnglandImperial Coll, Hammersmith Hosp, Dept Hematol, Fac Med, Du Cane Rd, London W12 0NN, England
Nadal, Elisabet
Longinotti, Maurizio
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机构:
Univ Sassari, Inst Hematol, I-07100 Sassari, ItalyImperial Coll, Hammersmith Hosp, Dept Hematol, Fac Med, Du Cane Rd, London W12 0NN, England
Longinotti, Maurizio
Dazzi, Francesco
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Imperial Coll, Hammersmith Hosp, Dept Hematol, Fac Med, Du Cane Rd, London W12 0NN, EnglandImperial Coll, Hammersmith Hosp, Dept Hematol, Fac Med, Du Cane Rd, London W12 0NN, England