Evaluation of a new high-throughput method for identifying quorum quenching bacteria

被引：60

作者：

Tang, Kaihao ^{[1
]}

Zhang, Yunhui ^{[1
]}

Yu, Min ^{[1
]}

Shi, Xiaochong ^{[1
]}

Coenye, Tom ^{[2
]}

Bossier, Peter ^{[3
]}

Zhang, Xiao-Hua ^{[1
]}

机构：

[1] Ocean Univ China, Coll Marine Life Sci, Qingdao 266003, Peoples R China

[2] Univ Ghent, Lab Pharmaceut Microbiol, B-9000 Ghent, Belgium

[3] Univ Ghent, Lab Aquaculture & Artemia Reference Ctr, B-9000 Ghent, Belgium

来源：

SCIENTIFIC REPORTS | 2013年 / 3卷

关键词：

LACTONE-DEGRADING BACTERIA; VIBRIO-HARVEYI; SENSING MOLECULES; CULTIVABLE BACTERIA; IDENTIFICATION; FISH; AUTOINDUCERS; RHIZOSPHERE; DIVERSITY; HOMOLOG;

D O I：

10.1038/srep02935

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Quorum sensing (QS) is a population-dependent mechanism for bacteria to synchronize social behaviors such as secretion of virulence factors. The enzymatic interruption of QS, termed quorum quenching (QQ), has been suggested as a promising alternative anti-virulence approach. In order to efficiently identify QQ bacteria, we developed a simple, sensitive and high-throughput method based on the biosensor Agrobacterium tumefaciens A136. This method effectively eliminates false positives caused by inhibition of growth of biosensor A136 and alkaline hydrolysis of N-acylhomoserine lactones (AHLs), through normalization of beta-galactosidase activities and addition of PIPES buffer, respectively. Our novel approach was successfully applied in identifying QQ bacteria among 366 strains and 25 QQ strains belonging to 14 species were obtained. Further experiments revealed that the QQ strains differed widely in terms of the type ofQQenzyme, substrate specificity and heat resistance. The QQ bacteria identified could possibly be used to control disease in aquaculture.

引用

页数：9

共 59 条

[1] The latent promiscuity of newly identified microbial lactonases is linked to a recently diverged phosphotriesterase [J].

Afriat, Livnat ;

Roodveldt, Cintia ;

Manco, Giuseppe ;

Tawfik, Dan S. .

BIOCHEMISTRY, 2006, 45 (46) :13677-13686

[2] Bacteriostatic anti-Vibrio parahaemolyticus activity of Pseudoalteromonas sp strains DIT09, DIT44 and DIT46 isolated from Southern Chilean intertidal Perumytilus purpuratus [J].

Aranda, Carlos P. ;

Valenzuela, Cristian ;

Barrientos, Javier ;

Paredes, Javier ;

Leal, Pablo ;

Maldonado, Miguel ;

Godoy, Felix A. ;

Osorio, Carlos G. .

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY, 2012, 28 (06) :2365-2374

[3] Adhesion to sole, Solea senegalensis Kaup, mucus of microorganisms isolated from farmed fish, and their interaction with Photobacterium damselae subsp piscicida [J].

Chabrillón, M ;

Rico, RM ;

Balebona, MC ;

Moriñigo, MA .

JOURNAL OF FISH DISEASES, 2005, 28 (04) :229-237

[4] Characterization of N-acylhomoserine lactone-degrading bacteria associated with the Zingiber officinale (ginger) rhizosphere: Co-existence of quorum quenching and quorum sensing in Acinetobacter and Burkholderia [J].

Chan, Kok-Gan ;

Atkinson, Steve ;

Mathee, Kalai ;

Sam, Choon-Kook ;

Chhabra, Siri Ram ;

Camara, Miguel ;

Koh, Chong-Lek ;

Williams, Paul .

BMC MICROBIOLOGY, 2011, 11

[5] A novel medium for the isolation of N-acylhomoserine lactone-degrading bacteria [J].

Chan, Kok-Gan ;

Yin, Wai-Fong ;

Sam, Choon-Kook ;

Koh, Chong-Lek .

JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, 2009, 36 (02) :247-251

[6] Isolation and identification of quorum quenching bacteria from environmental samples [J].

Christiaen, Steven E. A. ;

Brackman, Gilles ;

Nelis, Hans J. ;

Coenye, Tom .

JOURNAL OF MICROBIOLOGICAL METHODS, 2011, 87 (02) :213-219

[7] VanT, a homologue of Vibrio harveyi LuxR, regulates serine, metalloprotease, pigment, and biofilm production in Vibrio anguillarum [J].

Croxatto, A ;

Chalker, VJ ;

Lauritz, J ;

Jass, J ;

Hardman, A ;

Williams, P ;

Cámara, M ;

Milton, DL .

JOURNAL OF BACTERIOLOGY, 2002, 184 (06) :1617-1629

[8] Diversity of N-acyl homoserine lactone-producing and -degrading bacteria in soil and tobacco rhizosphere [J].

d'Angelo-Picard, C ;

Faure, D ;

Penot, I ;

Dessaux, Y .

ENVIRONMENTAL MICROBIOLOGY, 2005, 7 (11) :1796-1808

[9] The involvement of cell-to-cell signals in the development of a bacterial biofilm [J].

Davies, DG ;

Parsek, MR ;

Pearson, JP ;

Iglewski, BH ;

Costerton, JW ;

Greenberg, EP .

SCIENCE, 1998, 280 (5361) :295-298

[10] Autoinducers extracted from microbial mats reveal a surprising diversity of N-acylhomoserine lactones (AHLs) and abundance changes that may relate to diel pH [J].

Decho, Alan W. ;

Visscher, Pieter T. ;

Ferry, John ;

Kawaguchi, Tomohiro ;

He, Lijian ;

Przekop, Kristen M. ;

Norman, R. Sean ;

Reid, R. Pamela .

ENVIRONMENTAL MICROBIOLOGY, 2009, 11 (02) :409-420

← 1 2 3 4 5 6 →