Subnuclear compartmentalization of immunoglobulin loci during lymphocyte development

被引：566
作者：
Kosak, ST
Skok, JA
Medina, KL
Riblet, R
Le Beau, MM
Fisher, AG
Singh, H
机构：
[1] Univ Chicago, Dept Mol Genet & Cell Biol, Howard Hughes Med Inst, Chicago, IL 60637 USA
[2] Univ Chicago, Dept Med, Chicago, IL 60637 USA
[3] Hammersmith Hosp, Imperial Coll Sch Med, MRC, Clin Sci Ctr,Lymphocyte Dev Grp, London W12 0NN, England
[4] Torrey Pines Inst Mol Studies, San Diego, CA 92121 USA
来源：
SCIENCE | 2002年 / 296卷 / 5565期
关键词：
D O I：
10.1126/science.1068768
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Immunoglobulin (Ig) loci are selectively activated for transcription and rearrangement during B lymphocyte development. Using fluorescence in situ hybridization, we show that Ig heavy (H) and Igkappa Loci are preferentially positioned at the nuclear periphery in hematopoietic progenitors and pro-T cells but are centrally configured in pro-B nuclei. The inactive loci at the periphery do not associate with centromeric heterochromatin. Upon localization away from the nuclear periphery in pro-B cells, the IgH locus appears to undergo large-scale compaction. We suggest that subnuclear positioning represents a novel means of regulating transcription and recombination of IgH and Igkappa loci during lymphocyte development.
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页码：158 / 162
页数：5
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