Activity-guided Isolation of a Novel Protein from Croton tiglium with Antifungal and Antibacterial Activities

被引：12

作者：

Shahid, Muhammad ^{[2
]}

Tayyab, Muhammad ^{[2
]}

Naz, Farah ^{[2
]}

Jamil, Amer ^{[2
]}

Ashraf, Muhammad ^{[3
]}

Gilani, Anwarul Hassan ^{[1
]}

机构：

[1] Aga Khan Univ, Coll Med, Dept Biol & Biomed Sci, Nat Prod Res Div, Karachi, Pakistan

[2] Univ Agr Faisalabad, Dept Chem & Biochem, Mol Biochem Lab, Faisalabad 38040, Pakistan

[3] Univ Agr Faisalabad, Dept Bot, Faisalabad 38040, Pakistan

来源：

PHYTOTHERAPY RESEARCH | 2008年 / 22卷 / 12期

关键词：

Croton tiglium; bioactive peptide/protein; antibacterial; antifungal;

D O I：

10.1002/ptr.2544

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

This study describes the activity-guided isolation and purification of a novel antimicrobial protein from the seed of Croton tiglium Linn. Purification was carried out by (NH4)(2)SO4 precipitation, gel filtration and DEAE-cellulose ion-exchange chromatography. Antifungal and antibacterial activities were determined after each purification step. SDS-polyacrylamide gel electrophoresis revealed that the purified protein was a monomer with molecular mass of 50 kDa. This is a first report on purification of a protein from Croton tiglium, which possesses a strong and broad spectrum antimicrobial activity. Copyright (C) 2008 John Wiley & Sons, Ltd.

引用

页码：1646 / 1649

页数：4

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