Preparation, characterization, and cellular uptake of resveratrol-loaded trimethyl chitosan nanoparticles

被引：36
作者：
Min, Jeong Bin [1 ]
Kim, Eun Suh [1 ]
Lee, Ji-Soo [1 ]
Lee, Hyeon Gyu [1 ]
机构：
[1] Hanyang Univ, Dept Food & Nutr, 222 Wangsimni Ro, Seoul 04763, South Korea
来源：
FOOD SCIENCE AND BIOTECHNOLOGY | 2018年 / 27卷 / 02期
基金：
新加坡国家研究基金会;
关键词：
Trimethyl chitosan; Nanoencapsulation; Controlled release; Cellular uptake; Cytotoxicity; ORAL INSULIN DELIVERY; L-ASCORBIC-ACID; PHYSICOCHEMICAL PROPERTIES; COMPLEX COACERVATION; PROTEIN DELIVERY; IN-VITRO; STABILITY; ENCAPSULATION; ABSORPTION; CHLORIDE;
D O I：
10.1007/s10068-017-0272-2
中图分类号：
TS2 [食品工业];
学科分类号：
0832 ;
摘要：
The aim of the study was to encapsulate resveratrol (RV) in trimethyl chitosan (TMC) nanoparticles cross-linked with tripolyphosphate (TPP) and/or alginate to achieve controlled release and improved cellular uptake. TMC (degree of quaternization of 78%) was prepared by reacting purified chitosan with iodomethane. Three types of RV-loaded TMC nanoparticles were prepared: TMC-TPP (TP-NPs), TMC-alginate (TA-NPs), and TMC-alginate-TPP (TAP-NPs). TA-NPs and TAP-NPs showed lower particle size and encapsulation efficiency (EE), better distribution, and more sustained release than TP-NPs due to the high molecular weight and viscous property of alginate. Caco-2 cellular uptake of RV was improved by TMC nanoencapsulation, and TP-NPs showed the highest uptake due to its significantly higher EE. Compared with TAP-NPs, TA-NPs with higher positive surface charge showed higher cellular uptake. Moreover, Caco-2 cell growth-inhibiting activity of RV was significantly increased by TMC nanoencapsulation and TP-NPs showed the significantly highest activity with a good agreement with the permeability results.
引用
收藏
页码：441 / 450
页数：10
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